The protein stannin binds 14-3-3ζ and modulates mitogen-activated protein kinase signaling

被引:9
作者
Davidson, CE
Reese, BE
Billingsley, ML
Yun, JK
机构
[1] Penn State Univ, Milton S Hershey Med Ctr, Dept Pharmacol H078, Hershey, PA 17033 USA
[2] Penn State Univ, Milton S Hershey Med Ctr, Jake Gittlen Canc Ins, Hershey, PA 17033 USA
来源
MOLECULAR BRAIN RESEARCH | 2005年 / 138卷 / 02期
关键词
stannin; ERK/MAPK; p38/MAPK; 14-3-3; protein;
D O I
10.1016/j.molbrainres.2005.04.018
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The molecular mechanisms underlying the selective toxicity of trimethyltin (TMT) remain unclear. Stannin (Snn), a protein preferentially expressed in TMT-sensitive cells, provides a direct link to the molecular basis for TMT toxicity. Recent evidence demonstrated that Snn peptides bind and de-alkylate TMT to dimethyltin (DMT); Snn may mediate both TMT and DMT toxicity. In this study, we demonstrate that Snn co-immunoprecipitates with a scaffolding protein 14-3-3, specifically with 14-3-3 isotype. Consistent with this, a detailed amino acid sequence analysis shows that Snn contains a putative 14-3-3 protein-binding site located within its hydrophilic loop. In addition, we present the evidence that Snn overexpression results in reduced extracellular regulated kinase activation and increased p38 activation. In contrast, the activity of c-Jun N-terminal kinase did not change following Snn overexpression. This is the first evidence that demonstrates a direct interaction between Snn and MAPK signaling molecules. Together, these findings indicate a role of Snn in modulation of MAPK signaling pathways through its interactions with 14-3-3 xi. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:256 / 263
页数:8
相关论文
共 28 条
[1]   Mutation of BAD within the BH3 domain impairs its phosphorylation-mediated regulation [J].
Adachi, M ;
Zhang, YB ;
Imai, K .
FEBS LETTERS, 2003, 551 (1-3) :147-152
[2]   14-3-3 proteins in the nervous system [J].
Berg, D ;
Holzmann, C ;
Riess, O .
NATURE REVIEWS NEUROSCIENCE, 2003, 4 (09) :752-762
[3]   ACUTE TRIMETHYLTIN LIMBIC-CEREBELLAR SYNDROME [J].
BESSER, R ;
KRAMER, G ;
THUMLER, R ;
BOHL, J ;
GUTMANN, L ;
HOPF, HC .
NEUROLOGY, 1987, 37 (06) :945-950
[4]   Dealkylation of organotin compounds by biological dithiols: Toward the chemistry of organotin toxicity [J].
Buck, B ;
Mascioni, A ;
Que, L ;
Veglia, G .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (44) :13316-13317
[5]   Stannin, a protein that localizes to the mitochondria and sensitizes NIH-3T3 cells to trimethyltin and dimethyltin toxicity [J].
Davidson, CE ;
Reese, BE ;
Billingsley, ML ;
Yun, JK .
MOLECULAR PHARMACOLOGY, 2004, 66 (04) :855-863
[6]   Localization and characterization of stannin: Relationship to cellular sensitivity to organotin compounds [J].
Dejneka, NS ;
Patanow, CM ;
Polavarapu, R ;
Toggas, SM ;
Krady, JK ;
Billingsley, ML .
NEUROCHEMISTRY INTERNATIONAL, 1997, 31 (06) :801-815
[7]   Chromosomal localization and characterization of the stannin (Snn) gene [J].
Dejneka, NS ;
Polavarapu, R ;
Deng, X ;
Martin-DeLeon, PA ;
Billingsley, ML .
MAMMALIAN GENOME, 1998, 9 (07) :556-564
[8]   Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation [J].
Goldman, EH ;
Chen, L ;
Fu, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (11) :10442-10449
[9]   The neurotoxicant trimethyltin induces apoptosis via caspase activation, p38 protein kinase, and oxidative stress in PC12 cells [J].
Jenkins, SM ;
Barone, S .
TOXICOLOGY LETTERS, 2004, 147 (01) :63-72
[10]   USE OF AVIDIN-BIOTIN SUBTRACTIVE HYBRIDIZATION TO CHARACTERIZE MESSENGER-RNA COMMON TO NEURONS DESTROYED BY THE SELECTIVE NEUROTOXICANT TRIMETHYLTIN [J].
KRADY, JK ;
OYLER, GA ;
BALABAN, CD ;
BILLINGSLEY, ML .
MOLECULAR BRAIN RESEARCH, 1990, 7 (04) :287-297