Changes in the functional activity of mitochondria isolated from the liver of rat that passed the preadaptation to ultra-low deuterium concentration

被引：11

作者：

Dzhimak, S. S. ^{[1
,2
]}

Basov, A. A. ^{[1
,3
]}

Volchenko, N. N. ^{[1
]}

Samkov, A. A. ^{[1
]}

Fedulova, L. V. ^{[2
]}

Baryshev, M. G. ^{[1
]}

机构：

[1] Kuban State Univ, Krasnodar 350040, Russia

[2] Gorbatov All Russia Res Inst Meat Ind, Moscow 109316, Russia

[3] Kuban State Med Univ, Krasnodar 350063, Russia

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2017年 / 476卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

EXCHANGE-REACTIONS; WATER;

D O I：

10.1134/S1607672917050088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The incubation in deuterium-depleted medium of mitochondria isolated from the liver of rats that consumed drinking diet with depleted deuterium (46 ppm) revealed a higher (by 35%) generation of hydrogen peroxide in comparison with the mitochondria (isolated from the liver of rats that consumed drinking diet with 152 ppm deuterium) incubated in medium that contained 152 ppm deuterium. Succinate addition to the reaction system led to an increase in the production of hydrogen peroxide in isolated mitochondria by 44-81%, whereas the difference in the generation of H2O2 between the organelles incubated in mediums 46 and 152 ppm was reduced by 14%. The revealed change in the functional activity of mitochondria suggests the ability of the organism to adapt to the deuterium-depleted drinking diet, which is probably due to the formation of the D/H isotope transmembrane gradient.

引用

页码：323 / 325

页数：3

共 14 条

[1] NMR, EPR, and mass spectroscopy estimates of the antiradical activity of water with modified isotope composition [J].

Baryshev, M.G. ;

Basov, A.A. ;

Bolotin, S.N. ;

Dzhimak, S.S. ;

Kashaev, D.V. ;

Fedosov, S.R. ;

Frolov, V. Yu ;

Shashkov, D.I. ;

Lysak, D.A. ;

Timakov, A.A. .

Bulletin of the Russian Academy of Sciences: Physics, 2012, 76 (12) :1349-1352

[2]

Baryshev M.G., 2013, ECOLOGICAL B RES CTR, P13

[3] Submolecular regulation of cell transformation by deuterium depleting water exchange reactions in the tricarboxylic acid substrate cycle [J].

Boros, Laszlo G. ;

D'Agostino, Dominic P. ;

Katz, Howard E. ;

Roth, Justine P. ;

Meuillet, Emmanuelle J. ;

Somlyai, Gabor .

MEDICAL HYPOTHESES, 2016, 87 :69-74

[4] Content of deuterium in biological fluids and organs: Influence of deuterium depleted water on D/H gradient and the process of adaptation [J].

Dzhimak, S. S. ;

Basov, A. A. ;

Baryshev, M. G. .

DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2015, 465 (01) :370-373

[5] Application of NMR Spectroscopy to the Determination of Low Concentrations of Nonradioactive Isotopes in Liquid Media [J].

Dzhimak, S. S. ;

Basov, A. A. ;

Kopytov, G. F. ;

Kashaev, D. V. ;

Sokolov, M. E. ;

Artsybasheva, O. M. ;

Sharapov, K. S. ;

Baryshev, M. G. .

RUSSIAN PHYSICS JOURNAL, 2015, 58 (07) :923-929

[6] Correction of metabolic processes in rats during chronic endotoxicosis using isotope (D/H) exchange reactions [J].

Dzhimak, S. S. ;

Basov, A. A. ;

Fedulova, L. V. ;

Didikin, A. S. ;

Bikov, I. M. ;

Arcybasheva, O. M. ;

Naumov, G. N. ;

Baryshev, M. G. .

BIOLOGY BULLETIN, 2015, 42 (05) :440-448

[7]

Johnson D., 1967, Methods Enzymol., V10, P94, DOI [10.1016/0076-6879(67)10018-9, DOI 10.1016/0076-6879(67)10018-9]

[8]

Kirkina A. A., 2014, BIOPHYSICS-USSR, V59, P326

[9] DEUTERIUM DEPLETION INDUCES ANXIOLYTIC-LIKE EFFECTS IN RATS [J].

Mladin, Cristian ;

Ciobica, Alin ;

Lefter, Radu ;

Popescu, Alexandru ;

Bild, Walther .

ARCHIVES OF BIOLOGICAL SCIENCES, 2014, 66 (02) :947-953

[10]

Nikitin DI, 2003, BIOFIZIKA+, V48, P678

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