Virologic Features of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Children

被引：0

作者：

Yonker, Lael M. ^{[1
,2
,3
]}

Boucau, Julie ^{[4
]}

Regan, James ^{[5
]}

Choudhary, Manish C. ^{[3
,5
]}

Burns, Madeleine D. ^{[1
]}

Young, Nicola ^{[1
]}

Farkas, Eva J. ^{[1
]}

Davis, Jameson P. ^{[1
]}

Moschovis, Peter P. ^{[2
,3
]}

Kinane, T. Bernard ^{[2
,3
]}

Fasano, Alessio ^{[1
,2
,3
]}

Neilan, Anne M. ^{[2
,3
,6
]}

Li, Jonathan Z. ^{[3
,5
]}

Barczak, Amy K. ^{[3
,4
,6
]}

机构：

[1] Massachusetts Gen Hosp, Mucosal Immunol & Biol Res Ctr, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Dept Pediat, Boston, MA 02114 USA

[3] Harvard Med Sch, Boston, MA 02115 USA

[4] MIT & Harvard, Massachusetts Gen Hosp, Ragon Inst, Cambridge, MA USA

[5] Brigham & Womens Hosp, Dept Med, 75 Francis St, Boston, MA 02115 USA

[6] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2021年 / 224卷 / 11期

关键词：

SARS-CoV-2; pediatric COVID-19; viral dynamics;

D O I：

10.1093/infdis/jiab509

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Data on pediatric coronavirus disease 2019 (COVID-19) has lagged behind adults throughout the pandemic. An understanding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral dynamics in children would enable data-driven public health guidance. Methods. Respiratory swabs were collected from children with COVID-19. Viral load was quantified by reverse-transcription polymerase chain reaction (RT-PCR); viral culture was assessed by direct observation of cytopathic effects and semiquantitative viral titers. Correlations with age, symptom duration, and disease severity were analyzed. SARS-CoV-2 whole genome sequences were compared with contemporaneous sequences. Results. One hundred ten children with COVID-19 (median age, 10 years [range, 2 weeks-21 years]) were included in this study. Age did not impact SARS-CoV-2 viral load. Children were most infectious within the first 5 days of illness, and severe disease did not correlate with increased viral loads. Pediatric SARS-CoV-2 sequences were representative of those in the community and novel variants were identified. Conclusions. Symptomatic and asymptomatic children can carry high quantities of live, replicating SARS-CoV-2, creating a potential reservoir for transmission and evolution of genetic variants. As guidance around social distancing and masking evolves following vaccine uptake in older populations, a clear understanding of SARS-CoV-2 infection dynamics in children is critical for rational development of public health policies and vaccination strategies to mitigate the impact of COVID-19.

引用

页码：1821 / 1829

页数：9