Study on the interaction between bovine serum albumin and starch nanoparticles prepared by isoamylolysis and recrystallization

被引：11

作者：

Ji, Na ^{[1
]}

Qiu, Chao ^{[1
]}

Li, Xiaojing ^{[1
]}

Xiong, Liu ^{[1
]}

Sun, Qingjie ^{[1
]}

机构：

[1] Qingdao Agr Univ, Sch Food Sci & Engn, Qingdao 266109, Shandong, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 128卷

关键词：

Starch nanoparticles; Bovine serum albumin; Interaction; Spectroscopy; GOLD NANOPARTICLES; QUANTUM DOTS; FLUORESCENCE; PROTEIN; MEDIA; ZNS;

D O I：

10.1016/j.colsurfb.2015.03.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The current study primarily investigated the interaction of bovine serum albumin (BSA) with starch nanoparticles (SNPs) prepared by isoamylolysis and recrystallization using UV-vis, fluorescence, transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and circular dichroism (CD). The enhanced absorbance observed by UV-vis spectroscopy and decreased intensity of fluorescence spectroscopy suggested that BSA could bind to SNPs and form a BSA-SNP complex. The synchronous fluorescence spectra revealed that the emission maximum,of Tyr residue (at Delta lambda = 15 nm) was red-shifted at the investigated concentrations range, indicating that the conformation of BSA was changed. Quenching parameters showed that the quenching effect of SNPs was static quenching. TEM images showed that the SNPs were surrounded by protein coronae, indicating that nanoparticle-protein complexes had formed. The FTIR and CD characterization indicated that the SNPs induced structural changes in the secondary structure of BSA. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：594 / 599

页数：6

共 42 条

[1] Nanoarchitectonics: A Conceptual Paradigm for Design and Synthesis of Dimension-Controlled Functional Nanomaterials [J].

Ariga, Katsuhiko ;

Li, Mao ;

Richards, Gary J. ;

Hill, Jonathan P. .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2011, 11 (01) :1-13

[2] Steady state, time resolved, and circular dichroism spectroscopic studies to reveal the nature of interactions of zinc oxide nanoparticles with transport protein bovine serum albumin and to monitor the possible protein conformational changes [J].

Bardhan, Munmun ;

Mandal, Gopa ;

Ganguly, Tapan .

JOURNAL OF APPLIED PHYSICS, 2009, 106 (03)

[3] Systematic investigation on the interaction of bovine serum albumin with ZnO nanoparticles using fluorescence spectroscopy [J].

Bhogale, A. ;

Patel, N. ;

Sarpotdar, P. ;

Mariam, J. ;

Dongre, P. M. ;

Miotello, A. ;

Kothari, D. C. .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 102 :257-264

[4] ZnO nanoparticle-protein interaction: Corona formation with associated unfolding [J].

Bhunia, A. K. ;

Samanta, P. K. ;

Saha, S. ;

Kamilya, T. .

APPLIED PHYSICS LETTERS, 2013, 103 (14)

[5] A fluorescence spectroscopic study of the interaction between Glipizide and bovine serum albumin and its analytical application [J].

Cao, Shina ;

Liu, Baosheng ;

Li, Zhiyun ;

Chong, Baohong .

JOURNAL OF LUMINESCENCE, 2014, 145 :94-99

[6] Characterization of Silver/Bovine Serum Albumin (Ag/BSA) nanoparticles structure: Morphological, compositional, and interaction studies [J].

Gebregeorgis, A. ;

Bhan, C. ;

Wilson, O. ;

Raghavan, D. .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2013, 389 :31-41

[7] Combined fluorescence spectroscopy and molecular modeling studies on the interaction between harmalol and human serum albumin [J].

Hemmateenejad, Bahram ;

Shamsipur, Mojtaba ;

Samari, Fayezeh ;

Khayamian, Taghi ;

Ebrahimi, Malihe ;

Rezaei, Zahra .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2012, 67-68 :201-208

[8] Nanoparticle size matters in the formation of plasma protein coronas on Fe3O4 nanoparticles [J].

Hu, Zhengyan ;

Zhang, Hongyan ;

Zhang, Yi ;

Wu, Ren'an ;

Zou, Hanfa .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 121 :354-361

[9] Effective insulin delivery using starch nanoparticles as a potential trans-nasal mucoadhesive carrier [J].

Jain, Akhlesh Kumar ;

Khar, Roop K. ;

Ahmed, Farhan J. ;

Diwan, Prakash V. .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2008, 69 (02) :426-435

[10] Study on the binding of colloidal zinc oxide nanoparticles with bovine serum albumin [J].

Kathiravan, A. ;

Paramaguru, G. ;

Renganathan, R. .

JOURNAL OF MOLECULAR STRUCTURE, 2009, 934 (1-3) :129-137

← 1 2 3 4 5 →