Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

被引：102

作者：

Ghaffari, Maryam ^{[1
,2
,3
]}

Dehghan, Gholamreza ^{[2
]}

Baradaran, Behzad ^{[4
]}

Zarebkohan, Amir ^{[5
]}

Mansoori, Behzad ^{[4
]}

Soleymani, Jafar ^{[6
]}

Dolatabadi, Jafar Ezzati Nazhad ^{[7
]}

Hamblin, Michael R. ^{[8
,9
,10
]}

机构：

[1] Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran

[2] Univ Tabriz, Fac Nat Sci, Dept Biol, Tabriz, Iran

[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[4] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran

[5] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Med Nanotechnol, Tabriz, Iran

[6] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz, Iran

[7] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[8] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA

[9] Harvard Med Sch, Dept Dermatol, Boston, MA 02115 USA

[10] Univ Johannesburg, Fac Hlth Sci, Laser Res Ctr, ZA-2028 Doornfontein, South Africa

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2020年 / 188卷

关键词：

Curcumin; Bcl-2; siRNA; PAMAM dendrimer; Co-delivery; HeLa cancer cells; Nanoparticles; DENDROSOMAL CURCUMIN; ANTICANCER ACTIVITY; INDUCE APOPTOSIS; BREAST-CANCER; IN-VITRO; NANOPARTICLES; DRUG; DOXORUBICIN; CISPLATIN; PATHWAYS;

D O I：

10.1016/j.colsurfb.2019.110762

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a poly-amidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of similar to 180 nm, and high Cur loading content of similar to 82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.

引用

页数：11

共 49 条

[1] PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy [J].