Identification of circulating tumor DNA using a targeted 545-gene next generation sequencing panel in patients with gastric cancer

被引：5

作者：

Lan, Jing ^{[1
]}

Lu, Yaping ^{[2
]}

Guan, Yanfang ^{[2
]}

Chang, Lianpeng ^{[2
]}

Yu, Zhengyuan ^{[3
]}

Qian, Haixin ^{[1
]}

机构：

[1] Soochow Univ, Dept Gen Surg, Affiliated Hosp 1, 899 Pinghai Rd, Suzhou 215006, Jiangsu, Peoples R China

[2] Geneplus Beijing Inst, Dept Res & Dev, Beijing 102206, Peoples R China

[3] Soochow Univ, Dept Oncol, Affiliated Hosp 1, 899 Pinghai Rd, Suzhou 215006, Jiangsu, Peoples R China

来源：

ONCOLOGY LETTERS | 2020年 / 19卷 / 03期

基金：

美国国家科学基金会;

关键词：

gastric cancer; noninvasive; ctDNA; targeted sequencing; HER2; MUTATIONAL LANDSCAPE; GENOMIC ALTERATIONS; EGFR MUTATIONS; HER2; BREAST; TRASTUZUMAB; INHIBITOR; PROGNOSIS;

D O I：

10.3892/ol.2020.11305

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Gastric cancer (GC) is characterized by unique genetic aberrations. Some of these mutations may be used to predict tumor prognosis or to guide patient therapy. Cell-free circulating tumor DNA (ctDNA) has been considered a promising alternative to biopsy to identify genome aberrations. However, no standardized methods to detect ctDNA variations in patients with GC are currently available. In the present study, the targeted sequencing of 545 genes was used to identify somatic alterations in tissues and matched plasma samples of nine patients with GC. Driver gene mutations were detected in matched tissues and plasma ctDNA. The mutated reads concordance rate of ctDNA in GC tissues with matched tissues was 45%. A true positive copy number gain of human epidermal growth factor receptor 2 in plasma from patients with GC was identified. Furthermore, the ctDNA fraction in plasma cell-free DNA (cfDNA) was positively correlated with metastasis lymph node number and with lactate dehydrogenase level. In conclusion, results from the present study suggested that targeted sequencing of plasma ctDNA may be considered a potential option for the clinical monitoring of GC.

引用

页码：2251 / 2257

页数：7

共 39 条

[1] Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution [J].

Abbosh, Christopher ;

Birkbak, Nicolai J. ;

Wilson, Gareth A. ;

Jamal-Hanjani, Mariam ;

Constantin, Tudor ;

Salari, Raheleh ;

Le Quesne, John ;

Moore, David A. ;

Veeriah, Selvaraju ;

Rosenthal, Rachel ;

Marafioti, Teresa ;

Kirkizlar, Eser ;

Watkins, Thomas B. K. ;

McGranahan, Nicholas ;

Ward, Sophia ;

Martinson, Luke ;

Riley, Joan ;

Fraioli, Francesco ;

Al Bakir, Maise ;

Gronroos, Eva ;

Zambrana, Francisco ;

Endozo, Raymondo ;

Bi, Wenya Linda ;

Fennessy, Fiona M. ;

Sponer, Nicole ;

Johnson, Diana ;

Laycock, Joanne ;

Shafi, Seema ;

Czyzewska-Khan, Justyna ;

Rowan, Andrew ;

Chambers, Tim ;

Matthews, Nik ;

Turajlic, Samra ;

Hiley, Crispin ;

Lee, Siow Ming ;

Forster, Martin D. ;

Ahmad, Tanya ;

Falzon, Mary ;

Borg, Elaine ;

Lawrence, David ;

Hayward, Martin ;

Kolvekar, Shyam ;

Panagiotopoulos, Nikolaos ;

Janes, Sam M. ;

Thakrar, Ricky ;

Ahmed, Asia ;

Blackhall, Fiona ;

Summers, Yvonne ;

Hafez, Dina ;

Naik, Ashwini .

NATURE, 2017, 545 (7655) :446-+

[2]

Bang YJ, 2010, LANCET, V376, P1302

[3] Comprehensive molecular characterization of gastric adenocarcinoma [J].

Bass, Adam J. ;

Thorsson, Vesteinn ;

Shmulevich, Ilya ;

Reynolds, Sheila M. ;

Miller, Michael ;

Bernard, Brady ;

Hinoue, Toshinori ;

Laird, Peter W. ;

Curtis, Christina ;

Shen, Hui ;

Weisenberger, Daniel J. ;

Schultz, Nikolaus ;

Shen, Ronglai ;

Weinhold, Nils ;

Keiser, David P. ;

Bowlby, Reanne ;

Sipahimalani, Payal ;

Cherniack, Andrew D. ;

Getz, Gad ;

Liu, Yingchun ;

Noble, Michael S. ;

Pedamallu, Chandra ;

Sougnez, Carrie ;

Taylor-Weiner, Amaro ;

Akbani, Rehan ;

Lee, Ju-Seog ;

Liu, Wenbin ;

Mills, Gordon B. ;

Yang, Da ;

Zhang, Wei ;

Pantazi, Angeliki ;

Parfenov, Michael ;

Gulley, Margaret ;

Piazuelo, M. Blanca ;

Schneider, Barbara G. ;

Kim, Jihun ;

Boussioutas, Alex ;

Sheth, Margi ;

Demchok, John A. ;

Rabkin, Charles S. ;

Willis, Joseph E. ;

Ng, Sam ;

Garman, Katherine ;

Beer, David G. ;

Pennathur, Arjun ;

Raphael, Benjamin J. ;

Wu, Hsin-Ta ;

Odze, Robert ;

Kim, Hark K. ;

Bowen, Jay .

NATURE, 2014, 513 (7517) :202-209

[4]

Chen K, 2009, NAT METHODS, V6, P677, DOI [10.1038/nmeth.1363, 10.1038/NMETH.1363]

[5] Mutational landscape of gastric adenocarcinoma in Chinese: Implications for prognosis and therapy [J].

Chen, Kexin ;

Yang, Da ;

Li, Xiangchun ;

Sun, Baocun ;

Song, Fengju ;

Cao, Wenfeng ;

Brat, Daniel J. ;

Gao, Zhibo ;

Li, Haixin ;

Liang, Han ;

Zhao, Yanrui ;

Zheng, Hong ;

Li, Miao ;

Buckner, Jan ;

Patterson, Scott D. ;

Ye, Xiang ;

Reinhard, Christoph ;

Bhathena, Anahita ;

Joshi, Deepa ;

Mischel, Paul S. ;

Croce, Carlo M. ;

Wang, Yi Michael ;

Raghavakaimal, Sreekumar ;

Li, Hui ;

Lu, Xin ;

Pan, Yang ;

Chang, Han ;

Ba, Sujuan ;

Luo, Longhai ;

Cavenee, Webster K. ;

Zhang, Wei ;

Hao, Xishan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (04) :1107-1112

[6] Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples [J].

Cibulskis, Kristian ;

Lawrence, Michael S. ;

Carter, Scott L. ;

Sivachenko, Andrey ;

Jaffe, David ;

Sougnez, Carrie ;

Gabriel, Stacey ;

Meyerson, Matthew ;

Lander, Eric S. ;

Getz, Gad .

NATURE BIOTECHNOLOGY, 2013, 31 (03) :213-219

[7] Molecular analysis of gastric cancer identifies subtypes associated with distinct clinical outcomes [J].

Cristescu, Razvan ;

Lee, Jeeyun ;

Nebozhyn, Michael ;

Kim, Kyoung-Mee ;

Ting, Jason C. ;

Wong, Swee Seong ;

Liu, Jiangang ;

Yue, Yong Gang ;

Wang, Jian ;

Yu, Kun ;

Ye, Xiang S. ;

Do, In-Gu ;

Liu, Shawn ;

Gong, Lara ;

Fu, Jake ;

Jin, Jason Gang ;

Choi, Min Gew ;

Sohn, Tae Sung ;

Lee, Joon Ho ;

Bae, Jae Moon ;

Kim, Seung Tae ;

Park, Se Hoon ;

Sohn, Insuk ;

Jung, Sin-Ho ;

Tan, Patrick ;

Chen, Ronghua ;

Hardwick, James ;

Kang, Won Ki ;

Ayers, Mark ;

Dai Hongyue ;

Reinhard, Christoph ;

Loboda, Andrey ;

Kim, Sung ;

Aggarwal, Amit .

NATURE MEDICINE, 2015, 21 (05) :449-U217

[8] Analysis of Circulating Tumor DNA to Monitor Metastatic Breast Cancer [J].

Dawson, Sarah-Jane ;

Tsui, Dana W. Y. ;

Murtaza, Muhammed ;

Biggs, Heather ;

Rueda, Oscar M. ;

Chin, Suet-Feung ;

Dunning, Mark J. ;

Gale, Davina ;

Forshew, Tim ;

Mahler-Araujo, Betania ;

Rajan, Sabrina ;

Humphray, Sean ;

Becq, Jennifer ;

Halsall, David ;

Wallis, Matthew ;

Bentley, David ;

Caldas, Carlos ;

Rosenfeld, Nitzan .

NEW ENGLAND JOURNAL OF MEDICINE, 2013, 368 (13) :1199-1209

[9] Gefitinib Treatment in EGFR Mutated Caucasian NSCLC Circulating-Free Tumor DNA as a Surrogate for Determination of EGFR Status [J].

Douillard, Jean-Yves ;

Ostoros, Gyula ;

Cobo, Manuel ;

Ciuleanu, Tudor ;

Cole, Rebecca ;

McWalter, Gael ;

Walker, Jill ;

Dearden, Simon ;

Webster, Alan ;

Milenkova, Tsveta ;

McCormack, Rose .

JOURNAL OF THORACIC ONCOLOGY, 2014, 9 (09) :1345-1353

[10] Broad, Hybrid Capture-Based Next-Generation Sequencing Identifies Actionable Genomic Alterations in Lung Adenocarcinomas Otherwise Negative for Such Alterations by Other Genomic Testing Approaches [J].

Drilon, Alexander ;

Wang, Lu ;

Arcila, Maria E. ;

Balasubramanian, Sohail ;

Greenbowe, Joel R. ;

Ross, Jeffrey S. ;

Stephens, Phil ;

Lipson, Doron ;

Miller, Vincent A. ;

Kris, Mark G. ;

Ladanyi, Marc ;

Rizvi, Naiyer A. .

CLINICAL CANCER RESEARCH, 2015, 21 (16) :3631-3639

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