A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development

被引：159

作者：

Li, Ruili ^{[1
,2
]}

Liu, Peng ^{[1
,2
]}

Wan, Yinglang ^{[1
]}

Chen, Tong ^{[1
]}

Wang, Qinli ^{[1
]}

Mettbach, Ursula ^{[3
]}

Baluska, Frantisek ^{[3
]}

Samaj, Jozef ^{[4
]}

Fang, Xiaohong ^{[5
]}

Lucas, William J. ^{[6
]}

Lin, Jinxing ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Univ Bonn, Dept Plant Cell Biol, Inst Cellular & Mol Bot, D-53115 Bonn, Germany

[4] Palacky Univ, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Olomouc 78301, Czech Republic

[5] Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China

[6] Univ Calif Davis, Dept Plant Biol, Coll Biol Sci, Davis, CA 95616 USA

来源：

PLANT CELL | 2012年 / 24卷 / 05期

基金：

中国国家自然科学基金;

关键词：

DETERGENT-RESISTANT MEMBRANES; LIPID RAFTS; PROTEOMIC ANALYSIS; ARTIFICIAL MICRORNAS; POLLEN TUBES; PLANT-CELLS; DYNAMICS; GENE; CYTOSKELETON; TRAFFICKING;

D O I：

10.1105/tpc.112.095695

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endocytosis is essential for the maintenance of protein and lipid compositions in the plasma membrane and for the acquisition of materials from the extracellular space. Clathrin-dependent and -independent endocytic processes are well established in yeast and animals; however, endocytic pathways involved in cargo internalization and intracellular trafficking remain to be fully elucidated for plants. Here, we used transgenic green fluorescent protein-flotillin1 (GFP-Flot1) Arabidopsis thaliana plants in combination with confocal microscopy analysis and transmission electron microscopy immunogold labeling to study the spatial and dynamic aspects of GFP-Flot1-positive vesicle formation. Vesicle size, as outlined by the gold particles, was; similar to 100 nm, which is larger than the 30-nm size of clathrin-coated vesicles. GFP-Flot1 also did not colocalize with clathrin light chain-mOrange. Variable-angle total internal reflection fluorescence microscopy also revealed that the dynamic behavior of GFP-Flot1-positive puncta was different from that of clathrin light chain-mOrange puncta. Furthermore, disruption of membrane microdomains caused a significant alteration in the dynamics of Flot1-positive puncta. Analysis of artificial microRNA Flot1 transgenic Arabidopsis lines established that a reduction in Flot1 transcript levels gave rise to a reduction in shoot and root meristem size plus retardation in seedling growth. Taken together, these findings support the hypothesis that, in plant cells, Flot1 is involved in a clathrin-independent endocytic pathway and functions in seedling development.

引用

页码：2105 / 2122

页数：18