Purpose To test a simple electroretinographic protocol on a representative sample of minipigs. Animal studied Minipig. Procedures Electroretinogram recordings were conducted on 162 healthy minipigs (81 males and 81 females) aged 46 months. After a 1.5-h light-adaptation period, the animals were anesthetized with general anesthesia. First, binocular full-field photopic electroretinogram recordings were conducted under photopic conditions. Subsequently, scotopic electroretinogram recordings were conducted during dark-adaptation periods every 4 min for a 20-min period. At the end of this period, the maximal combined rod-cone response was recorded by measuring the retinal response to a single high-intensity flash. We used sclerocorneal clip electrodes as active electrodes and needle electrodes as reference and ground electrodes. Results The a-wave and b-wave peak times and amplitudes have been measured and statistically analyzed. For each of the statistical comparisons, normality and homogeneity of variances were evaluated. No significant gender differences were observed, with the exception of a higher b-wave amplitude for the photopic ERG recordings observed in females when compared to males (48.14 +/- 12.909 mu V vs. 42.88 +/- 10.666 mu V; P = 0.005). The process of dark adaptation was evaluated, and the maximal combined rod-cone response was measured (a- and b-waves amplitude and peak time). Conclusions We conducted photopic and scotopic electroretinogram recordings from a protocol based on light adaptation followed by dark adaptation using sclerocorneal clip electrodes, which allows quick assembly and examination.
机构:
Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Harvard Med Sch, Ophthalmol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Akula, James D.
Ambrosio, Lucia
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Harvard Med Sch, Ophthalmol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Ambrosio, Lucia
Howard, Fiona I.
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Northeastern Univ, Psychol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Howard, Fiona I.
Hansen, Ronald M.
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Harvard Med Sch, Ophthalmol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Hansen, Ronald M.
Fulton, Anne B.
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Harvard Med Sch, Ophthalmol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
机构:
Singapore Eye Res Inst, Singapore, Singapore
Singapore Natl Eye Ctr, Singapore 168751, SingaporeSingapore Eye Res Inst, Singapore, Singapore
Chia, Audrey
Li, Wen
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Singapore Clin Res Inst, Singapore, Singapore
DUKE NUS Grad Med Sch, Ctr Quantitat Med, Singapore, SingaporeSingapore Eye Res Inst, Singapore, Singapore
Li, Wen
Tan, Donald
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Singapore Eye Res Inst, Singapore, Singapore
Singapore Natl Eye Ctr, Singapore, SingaporeSingapore Eye Res Inst, Singapore, Singapore
Tan, Donald
Luu, Chi D.
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Singapore Eye Res Inst, Singapore, Singapore
Univ Melbourne, Royal Victorian Eye & Ear Hosp, Ctr Eye Res Australia, Melbourne, Vic, AustraliaSingapore Eye Res Inst, Singapore, Singapore
机构:
Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Harvard Med Sch, Ophthalmol, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Akula, James D.
Ziska, Emma
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Northeastern Univ, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Ziska, Emma
Peralta, Jashwa
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Northeastern Univ, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Peralta, Jashwa
Manne, Rithvik
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Boston Childrens Hosp, Ophthalmol, Boston, MA USA
Northeastern Univ, Boston, MA 02115 USABoston Childrens Hosp, Ophthalmol, Boston, MA USA
Manne, Rithvik
Patel, Jagvi K.
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Boston Childrens Hosp, Ophthalmol, Boston, MA USABoston Childrens Hosp, Ophthalmol, Boston, MA USA