Alcohol induced structural and dynamic changes in β-lactoglobulin in aqueous solution: A neutron scattering study

被引:18
作者
Yoshida, Koji [1 ]
Vogtt, Karsten [2 ]
Izaola, Zunbeltz [2 ]
Russina, Margarita [2 ]
Yamaguchi, Toshio [1 ]
Bellissent-Funel, Marie-Claire [2 ,3 ]
机构
[1] Fukuoka Univ, Fac Sci, Dept Chem, Jonan Ku, Fukuoka 8140180, Japan
[2] Helmholtz Zentrum Berlin Mat & Energie GmbH, D-14109 Berlin, Germany
[3] CEA Saclay, Lab Leon Brillouin, CNRS, CEA, F-91191 Gif Sur Yvette, France
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2012年 / 1824卷 / 03期
关键词
beta-Lactoglobulin; Alcohol induced change; Small-angle neutron scattering; Quasi-elastic neutron scattering; SMALL-ANGLE NEUTRON; X-RAY-SCATTERING; TRYPSIN-INHIBITOR BPTI; EGG-WHITE LYSOZYME; THERMAL-DENATURATION; ALPHA-LACTALBUMIN; LIGHT-SCATTERING; INDUCED GELATION; IR SPECTROSCOPY; SANS-U;
D O I
10.1016/j.bbapap.2011.12.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structural and dynamic properties of beta-lactoglobulin (beta-LG) were revealed as a function of alcohol concentration in ethanol- and trifluoroethanol(TFE)-water mixtures with circular dichroism (CD), small-angle neutron scattering (SANS) and quasi-elastic neutron scattering (QENS). The CD spectra showed that an increase in TFE concentration promotes the formation of the beta-sheet structure of beta-LG. The SANS-intensities were fitted using form factors for two attached spheres for the native and native-like states of the protein. At higher alcohol concentrations, where aggregation takes place, a form factor modelling diffusion limited colloidal aggregation (DLCA) was employed. The QENS-data were analyzed in terms of internal motions for all alcohol concentrations. While low concentrations of TFE (10% (v/v)) lead to an increase of the mean square amplitudes of vibrations <u(2)> and a retention of a native-like structure - but not to an increase of the characteristic radius of proton diffusion processes a. Addition of 20% (v/v) of TFE induces aggregation, going along with a further increase of <u(2)>. Further increase of TFE concentration to 30% (v/v) changes the nanoscale structure of the oligomeric nucleate, but induces no further significant changes in <u(2)>. The present study underlines the necessity of methods sensitive to the dynamics of a system to obtain a complete picture of a molecular process. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:502 / 510
页数:9
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