The chlorogenic acids of Hemerocallis

The chlorogenic acids in a methanolic extract of freeze-dried Hemerocallis (Chinese day lily) have been qualitatively profiled by LC-MS3. Three caffeoylquinic acids (3-CQA (I), 4-CQA (III) and 5-CQA (II)), three p-coumaroylquinic acids (3-pCoQA (IV), 4-pCoQA (VI) and 5-pCoQA (V)) and two feruloylquinic acids (3-FQA (VII) and 4-FQA (IX)) have been identified. The dominance of the 3-acyl and 4-acyl CGA relative to the 5-acyl isomer is unusual and makes this material a convenient source of these commercially non-available chlorogenic acids. A minor revision has been made to the structure-diagnostic hierarchical key previously developed for characterising chlorogenic acids by LC-MSn. (c) 2005 Elsevier Ltd. All rights reserved.