Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson-Crick base pairing

被引:129
作者
Berger, Or [1 ]
Adler-Abramovich, Lihi [1 ]
Levy-Sakin, Michal [2 ]
Grunwald, Assaf [2 ]
Liebes-Peer, Yael [2 ]
Bachar, Mor [1 ]
Buzhansky, Ludmila [1 ]
Mossou, Estelle [3 ,4 ]
Forsyth, V. Trevor [3 ,4 ]
Schwartz, Tal [2 ]
Ebenstein, Yuval [2 ]
Frolow, Felix [1 ,5 ]
Shimon, Linda J. W. [6 ]
Patolsky, Fernando [2 ,7 ]
Gazit, Ehud [1 ,7 ]
机构
[1] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Ramat Aviv, Israel
[2] Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Ramat Aviv, Israel
[3] Inst Laue Langevin, Partnership Struct Biol, F-38042 Grenoble 9, France
[4] Keele Univ, Fac Nat Sci, Keele ST5 5BG, Staffs, England
[5] Tel Aviv Univ, George S Wise Fac Life Sci, Daniella Rich Inst Struct Biol, IL-69978 Ramat Aviv, Israel
[6] Weizmann Inst Sci, Dept Chem Res Support, IL-76100 Rehovot, Israel
[7] Tel Aviv Univ, Dept Mat Sci & Engn, Iby & Aladar Fleischman Fac Engn, IL-69978 Ramat Aviv, Israel
关键词
NANOSCALE SHAPES; FOLDING DNA; NANOTUBES; NANOFIBERS; PNA; HYDROGELS;
D O I
10.1038/nnano.2015.27
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The two main branches of bionanotechnology involve the self-assembly of either peptides or DNA. Peptide scaffolds offer chemical versatility, architectural flexibility and structural complexity, but they lack the precise base pairing and molecular recognition available with nucleic acid assemblies. Here, inspired by the ability of aromatic dipeptides to form ordered nanostructures with unique physical properties, we explore the assembly of peptide nucleic acids (PNAs), which are short DNA mimics that have an amide backbone. All 16 combinations of the very short di-PNA building blocks were synthesized and assayed for their ability to self-associate. Only three guanine-containing di-PNAs-CG, GC and GG-could form ordered assemblies, as observed by electron microscopy, and these di-PNAs efficiently assembled into discrete architectures within a few minutes. The X-ray crystal structure of the GC di-PNA showed the occurrence of both stacking interactions and Watson-Crick base pairing. The assemblies were also found to exhibit optical properties including voltage-dependent electroluminescence and wide-range excitation-dependent fluorescence in the visible region.
引用
收藏
页码:353 / 360
页数:8
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