Analysis of serum lipoproteins by high performance gel filtration chromatography

被引:0
作者
Kitamura, T [1 ]
Ito, S [1 ]
Kato, Y [1 ]
Sasamoto, K [1 ]
Okazaki, M [1 ]
机构
[1] TOKYO MED & DENT UNIV,DEPT GEN EDUC,CHEM LAB,CHIBA 272,JAPAN
关键词
analysis of serum lipoproteins; high-performance gel filtration chromatography; TSKgel Lipopropak; TSKeluent LP-1; total cholesterol and high density lipoprotein cholesterol;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We evaluated a new gel filtration column and eluent commercially available under the trade-names of TSKgel Lipopropak and TSKeluent LP-1 for the analysis of serum lipoproteins. Lipoproteins were separated within about 20 min, and were detected by post column reaction using the cholesterol oxidase-peroxidase system. Total and high density lipoprotein(HDL) cholesterols could be quantified every 10 min, with high recovery and high reproducibility.
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页码:103 / 106
页数:4
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