A slow-cycling subpopulation of melanoma cells with highly invasive properties

被引：57

作者：

Perego, M. ^{[1
]}

Maurer, M. ^{[2
]}

Wang, J. X. ^{[1
]}

Shaffer, S. ^{[3
]}

Mueller, A. C. ^{[4
]}

Parapatics, K. ^{[4
]}

Li, L. ^{[1
]}

Hristova, D. ^{[1
]}

Shin, S. ^{[1
]}

Keeney, F. ^{[1
]}

Liu, S. ^{[5
]}

Xu, X. ^{[5
]}

Raj, A. ^{[3
]}

Jensen, J. K. ^{[6
]}

Bennett, K. L. ^{[4
]}

Wagner, S. N. ^{[2
]}

Somasundaram, R. ^{[1
]}

Herlyn, M. ^{[1
]}

机构：

[1] Wistar Inst Anat & Biol, Melanoma Res Ctr, 3601 Spruce St, Philadelphia, PA 19104 USA

[2] Med Univ Vienna, Div Immunol Allergy & Infect Dis, Vienna, Austria

[3] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

[4] Austrian Acad Sci, CeMM Res Ctr Mol Med, Vienna, Austria

[5] Univ Penn, Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

[6] Aarhus Univ, Dept Mol Biol & Genet, Aarhus, Denmark

来源：

ONCOGENE | 2018年 / 37卷 / 03期

关键词：

BONE MORPHOGENETIC PROTEIN-1; PROTEASE NEXIN-1; PROGNOSTIC-SIGNIFICANCE; PLASMINOGEN-ACTIVATOR; SAMPLE PREPARATION; INITIATING CELLS; MESSENGER-RNA; LOW NUMBER; CANCER; EXPRESSION;

D O I：

10.1038/onc.2017.341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Melanoma is a heterogeneous tumor with different subpopulations showing different proliferation rates. Slow-cycling cells were previously identified in melanoma, but not fully biologically characterized. Using the label-retention method, we identified a subpopulation of slow-cycling cells, defined as label-retaining cells (LRC), with strong invasive properties. We demonstrate through live imaging that LRC are leaving the primary tumor mass at a very early stage and disseminate to peripheral organs. Through global proteome analyses, we identified the secreted protein SerpinE2/protease nexin-1 as causative for the highly invasive potential of LRC in melanomas.

引用

页码：302 / 312

页数：11

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