Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles

被引：26

作者：

Braz, Luis ^{[1
,2
,3
,4
]}

Grenha, Ana ^{[4
,5
]}

Corvo, Marta C. ^{[6
]}

Lourenco, Joao Paulo ^{[1
,7
,8
]}

Ferreira, Domingos ^{[3
]}

Sarmento, Bruno ^{[9
,10
,11
]}

Rosa da Costa, Ana M. ^{[1
,7
]}

机构：

[1] CIQA Ctr Res Chem Algarve, Fac Sci & Technol, Campus Gambelas, P-8005139 Faro, Portugal

[2] Univ Algarve, Ctr Estudos & Desenvolvimento Saude CESUAlg, Ave Dr Adelino da Palma Carlos, P-8000510 Faro, Portugal

[3] Univ Porto, Fac Pharm, Dept Pharmaceut Technol, Rua Jorge Viterbo Ferreira 228, P-4050313 Oporto, Portugal

[4] Univ Algarve, CBMR Ctr Biomed Res, Fac Sci & Technol, Campus Gambelas, P-8005139 Faro, Portugal

[5] Univ Algarve, CCMAR Ctr Marine Sci, Campus Gambelas, P-8005139 Faro, Portugal

[6] UNL, CENIMAT i3N, Dept Mat Sci, Fac Sci & Technol, P-2829516 Caparica, Portugal

[7] Univ Algarve, Fac Sci & Technol, Dept Chem & Pharm, Campus Gambelas, P-8005139 Faro, Portugal

[8] Univ Lisbon, Inst Super Tecn, CQE, Lisbon, Portugal

[9] Univ Porto, Inst Invest & Inovacao Saude, Rua Alfredo Allen 208, P-4200135 Oporto, Portugal

[10] Univ Porto, Biocarrier Grp, INEB, Inst Engn Biomed, Rua Alfredo Allen 208, P-4200135 Oporto, Portugal

[11] CESPU, Inst Invest & Formacao Avancada Ciencias & Tecnol, Rua Cent Gandra 1317, P-4585116 Gandra, Portugal

来源：

CARBOHYDRATE POLYMERS | 2018年 / 181卷

关键词：

Carboxylated locust bean gum; Polyelectrolyte complexation; Polymeric nanoparticles; Sulfated locust bean gum; Tetraalkylammonium locust bean gum; DRUG-DELIVERY; IN-VITRO; CHITOSAN/CARRAGEENAN NANOPARTICLES; CELLULAR UPTAKE; SURFACE-CHARGE; CHITOSAN; POLYSACCHARIDE; CYTOTOXICITY; RELEASE; GALACTOMANNANS;

D O I：

10.1016/j.carbpol.2017.11.052

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.

引用

页码：974 / 985

页数：12

共 68 条

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