Cobalamin reduction by dithionite. Evidence for the formation of a six-coordinate cobalamin(II) complex

被引：46

作者：

Salnikov, Denis S. ^{[1
,2
]}

Silaghi-Dumitrescu, Radu ^{[3
]}

Makarov, Sergei V. ^{[1
]}

van Eldik, Rudi ^{[2
]}

Boss, Gerry R. ^{[4
]}

机构：

[1] State Univ Chem & Technol, Ivanovo 153000, Russia

[2] Univ Erlangen Nurnberg, Dept Chem & Pharm, D-91058 Erlangen, Germany

[3] Univ Babes Bolyai, RO-400028 Cluj Napoca, Romania

[4] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

来源：

DALTON TRANSACTIONS | 2011年 / 40卷 / 38期

基金：

美国国家卫生研究院;

关键词：

NITRIC-OXIDE; CYTOCHROME-P450; VITAMIN-B-12; MECHANISM; KINETICS; BINDING;

D O I：

10.1039/c1dt10219b

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Evidence for the formation of a unique, six-coordinate cobalamin(II) complex with the anion-radical SO2- during the reduction of aquacobalamin(III) by sodium dithionite, was obtained from spectrophotometric and EPR measurements. The pK(a) value of the weakly coordinated dimethylbenzimidazole group was found to be 4.8 +/- 0.1 at 25 degrees C.

引用

页码：9831 / 9834

页数：4

共 19 条

[1] Nitric oxide scavenging by the cobalamin precursor cobinamide
Broderick, KE
Singh, V
Zhuang, SH
Kambo, A
Chen, JC
Sharma, VS
Pilz, RB
Boss, GR
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (10) : 8678 - 8685
[2] ACID-BASE PROPERTIES OF ALPHA-RIBAZOLE AND THE THERMODYNAMICS OF DIMETHYLBENZIMIDAZOLE ASSOCIATION IN ALKYLCOBALAMINS
BROWN, KL
HAKIMI, JM
NUSS, DM
MONTEJANO, YD
JACOBSEN, DW
[J]. INORGANIC CHEMISTRY, 1984, 23 (10) : 1463 - 1471
[3] HETERONUCLEAR NMR-STUDIES OF COBALAMINS .6. THE NUCLEOTIDE LOOP OF BASE-OFF COBALAMINS AND THE NATURE OF THE BASE-OFF SPECIES
BROWN, KL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (08) : 2277 - 2284
[4] Nitric oxide inhibits methionine synthase activity in vivo and disrupts carbon flow through the folate pathway
Danishpajooh, IO
Gudi, T
Chen, YC
Kharitonov, VG
Sharma, VS
Boss, GR
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (29) : 27296 - 27303
[5] SULPHITOCOBALAMIN
DOLPHIN, DH
JOHNSON, AW
SHAW, N
[J]. NATURE, 1963, 199 (488) : 170 - &
[6] Mechanistic Studies on the Reaction between R2N-NONOates and Aquacobalamin: Evidence for Direct Transfer of a Nitroxyl Group from R2N-NONOates to Cobalt(III) Centers
Hassanin, Hanaa A.
Hannibal, Luciana
Jacobsen, Donald W.
El-Shahat, Mohamed F.
Hamza, Mohamed S. A.
Brasch, Nicola E.
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (47) : 8909 - 8913
[7] CORPHYRINS
HILL, JA
WILLIAMS, RJP
PRATT, JM
[J]. JOURNAL OF THEORETICAL BIOLOGY, 1962, 3 (03) : 423 - +
[8] Kräutler B, 2005, BIOCHEM SOC T, V33, P806
[9] Spectroscopic and computational studies of Co1+ cobalamin:: Spectral and electronic properties of the "superreduced" B12 cofactor
Liptak, Mathew D.
Brunold, Thomas C.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (28) : 9144 - 9156
[10] Makarov S.V., 2001, Russ. Chem. Rev, V70, P885, DOI [10.1070/RC2001v070n10ABEH000659, DOI 10.1070/RC2001V070N10ABEH000659]

← 1 2 →