Extraction, Purification and Characterization of Thermostable, Alkaline Tolerant α-Amylase from Bacillus cereus

被引：38

作者：

Annamalai, N. ^{[1
]}

Thavasi, R. ^{[2
]}

Vijayalakshmi, S. ^{[1
]}

Balasubramanian, T. ^{[1
]}

机构：

[1] Annamalai Univ, CAS Marine Biol, Parangipettai 608502, India

[2] Polytech Univ, Metrotech Ctr 6, Dept Chem & Biol Sci, Brooklyn, NY 11201 USA

来源：

INDIAN JOURNAL OF MICROBIOLOGY | 2011年 / 51卷 / 04期

关键词：

Cuddalore harbour waters; Bacillus alpha-amylase; Thermostable; Alkaline tolerant; BIOCHEMICAL-CHARACTERIZATION; BETA-AMYLASE;

D O I：

10.1007/s12088-011-0160-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thermostable alkaline a-amylase producing bacterium Bacillus cereus strain isolated from Cuddalore harbour waters grew maximally in both shake flask and fermentor, and produced a-amylase at 35 degrees C, pH 7.5 and 1.0% of substrate concentrations. a-Amylase activity was maximum at 65 degrees C, pH 8.0, 89% of its activity was sustained even at pH 11.0. Added with MnCl2, alpha-amylase activity showed 4% increase but it was inhibited by EDTA. The molecular weight of the purified a-amylase is 42 kDa.

引用

页码：424 / 429

页数：6

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