Characterization of cyanide binding to cytochrome c oxidase immobilized in electrode-supported lipid bilayer membranes

被引：11
|
作者：
Su, LY
Kelly, JB
Hawkridge, FM
Rhoten, MC
Baskin, SI
机构：
[1] Virginia Commonwealth Univ, Dept Chem, Richmond, VA 23284 USA
[2] Longwood Univ, Dept Nat Sci, Farmville, VA 23909 USA
[3] USA, Med Res Inst Chem Def, Aberdeen Proving Ground, MD 21010 USA
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2005年 / 581卷 / 02期
基金：
美国国家科学基金会;
关键词：
cytochrome c oxidase; biosensor; cyanide dioxygen;
D O I：
10.1016/j.jelechem.2005.04.023
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Bovine cytochrome c oxidase has been successfully immobilized in elect rode-supported lipid bilayer membranes to investigate the effect of cyanide binding on the oxidation of ferrocytochrome c and the electroreduction of dioxygen. Cyanide binding to oxidase was found to be reversible and exhibited 1: 1 stoichiometry. Binding constants (K) were also determined for binding of cyanide to the reduced (62 mu M) and oxidized (195 mu M) forms of the oxidase. The cytochrome c oxidase-modified electrodes described here could potentially be used as an amperometric biosensor for the detection of cyanide. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：241 / 248
页数：8
相关论文
共 50 条
  • [31] Synthesis and characterization of supported lipid bilayer membranes from complex lipid mixtures
    Hardy, Gregory
    Shapter, Joe
    Alam, Munir
    Zauscher, Stefan
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253
  • [32] Tuning of lipid bilayer fluidity regulates mediated electron transfer reactions of glucose oxidase immobilized on lipid bilayer films on an electrode
    Tominaga, M
    Kusano, S
    Nakashima, N
    BIOELECTROCHEMISTRY AND BIOENERGETICS, 1997, 42 (01): : 59 - 62
  • [33] Ion channel behavior of supported bilayer lipid membranes on a glassy carbon electrode
    Wu, ZY
    Tang, JL
    Cheng, ZL
    Yang, XR
    Wang, EK
    ANALYTICAL CHEMISTRY, 2000, 72 (24) : 6030 - 6033
  • [34] Interactions of cytochrome C with reconstituted cytochrome C oxidase membranes. Evidence for increased cytochrome C oxidase boundary lipid induced by cytochrome C.
    Kleinschmidt, JH
    Powell, GL
    Marsh, D
    BIOPHYSICAL JOURNAL, 1997, 72 (02) : TH313 - TH313
  • [35] PROBING THE OXYGEN-BINDING SITE OF CYTOCHROME-C-OXIDASE BY CYANIDE
    WILSON, MT
    ANTONINI, G
    MALATESTA, F
    SARTI, P
    BRUNORI, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (39) : 24114 - 24119
  • [36] A NEW SPECTRAL INTERMEDIATE IN CYANIDE BINDING WITH THE OXIDIZED CYTOCHROME-C-OXIDASE
    BERKA, V
    VYGODINA, T
    MUSATOV, A
    NICHOLLS, P
    KONSTANTINOV, AA
    FEBS LETTERS, 1993, 315 (03) : 237 - 241
  • [37] HETEROGENEITY IN FAST CYANIDE-BINDING PREPARATIONS OF CYTOCHROME-C-OXIDASE
    MOODY, AJ
    RICH, PR
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (03) : S262 - S262
  • [38] PHOSPHATIDYLCHOLINE EXCHANGE BETWEEN BOUNDARY LIPID AND BILAYER DOMAINS IN CYTOCHROME-OXIDASE CONTAINING MEMBRANES
    JOST, PC
    NADAKAVUKAREN, KK
    GRIFFITH, OH
    BIOCHEMISTRY, 1977, 16 (14) : 3110 - 3114
  • [39] Complexation of cytochrome c with calixarenes incorporated into the lipid vesicles and supported membranes
    Garaiova, Zuzana
    Mohsin, Muhammad Ali
    Vargova, Veronika
    Banica, Florinel-Gabriel
    Hianik, Tibor
    BIOELECTROCHEMISTRY, 2012, 87 : 220 - 225
  • [40] Construction and characterization of soft-supported lipid bilayer membranes for biosensors application
    Jimenez, Jeffy
    Heim, August J., II
    Matthews, W. Garrett
    Alcantar, Norma
    2006 28TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-15, 2006, : 5512 - +
12345