Detection of human herpesviruses HHV-6, HHV-7 and HHV-8 in whole blood by real-time PCR using the new CMV, HHV-6, 7, 8 R-gene™ kit

被引：14

作者：

Deback, C. ^{[1
]}

Agbalika, F. ^{[2
,3
]}

Scieux, C. ^{[2
,3
]}

Marcelin, A. G. ^{[1
]}

Gautheret-Dejean, A. ^{[1
]}

Cherot, J. ^{[2
]}

Hermet, L. ^{[1
]}

Roger, O. ^{[1
]}

Agut, H. ^{[1
]}

机构：

[1] Univ Paris 06, UPRES EA 2387, CERVI, Serv Virol,Grp Hosp Pitie Salpetriere,AP HP, F-75013 Paris 13, France

[2] CHU St Louis, Microbiol Serv, AP HP, F-75475 Paris, France

[3] Univ Paris 07, Paris, France

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 149卷 / 02期

关键词：

human herpesvirus 6; human herpesvirus 7; human herpesvirus 8; real-time PCR; whole blood;

D O I：

10.1016/j.jviromet.2008.01.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) are lymphotropic herpesviruses that may cause opportunistic diseases in immuno-suppressed patients such as transplant or AIDS patients. The new commercial CMV HHV-6, 7, 8 R-gene (TM) kit (Argene, Varilhes, France) for the simultaneous quantitation of HHV-6 and qualitative detection of HHV-7 and HHV-8 was evaluated using whole blood samples (respectively, n = 175, 100 and 161) and using different extraction and real-time PCR platforms in two Centers A and B. In comparison with HHV-6 in-house real-time PCR the commercial kit showed agreements of 96% (n = 75) and 85% (n = 100) in A and B, respectively, with significant Spearman's correlation between both techniques (in A: r = 0.97 [p < 0.001]; in B: r = 0.70 [p < 0.001]). The Bland-Altman test results and prospective monitoring of patients confirmed the accuracy of these HHV-6 real-time PCR techniques. The agreement between the in-house HHV-7 PCR and commercial kit was of 86% (n = 100). In comparison with in-house HHV-8 real-time PCRs, the commercial kit showed agreements of 100% (n = 61) and 93.7% (n = 96) in A and B, respectively. These results demonstrate that the new commercial CMV HHV-6, 7, 8 R-gene (TM) kit was an efficient and reliable tool for the diagnosis of herpesvirus 6, 7, 8 infections. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：285 / 291

页数：7

共 28 条

[11] Detection of human Betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects [J].