The effect of K+ on caspase activity of corneal epithelial cells exposed to UVB

被引：5

作者：

Leerar, John R. ^{[1
]}

Glupker, Courtney D. ^{[1
]}

Schotanus, Mark P. ^{[1
]}

Ubels, John L. ^{[1
]}

机构：

[1] Calvin Coll, Dept Biol, 1726 Knollcrest Circle Dr SE, Grand Rapids, MI 49546 USA

来源：

EXPERIMENTAL EYE RESEARCH | 2016年 / 151卷

关键词：

Apoptosis; Cornea; Corneal epithelium; Potassium channels; Caspase; Ultraviolet; ULTRAVIOLET-B; APOPTOSIS; ACTIVATION; DEATH; INVOLVEMENT; PATHWAYS; EFFLUX;

D O I：

10.1016/j.exer.2016.07.014

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Exposure of human corneal limbal epithelial (HCLE) cells to UVB triggers rapid loss of K+ and apoptosis via activation of caspases -9, -8 and -3. It has been shown that preventing loss of intracellular K+ can inhibit apoptosis. The goal of this study was to investigate the effect of K+ on the UVB-induced caspase activity. HCLE cells were exposed to 150 mJ/cm(2) UVB, followed by measurement of caspase activity in cell lysates. Caspase activity was measured in the presence and absence of 100 mM K+ in the reaction buffer. UVB-induced activity of caspases -9, -8 and -3 all decreased in the presence of 100 mM K+. These results suggest that a role of high [K+] in the cell is to inhibit caspase activity. Therefore, when cells lose K+ in response to UVB, caspases are activated and cells go into apoptosis. This supports our hypothesis that K+ inhibits caspase activity. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：23 / 25

页数：3

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