Determination of total protein by spectrophotometry: Advantages and disadvantages of proposed methods.

被引：64
作者：
Zaia, DAM [1 ]
Zaia, CTBV
Lichtig, J
机构：
[1] Univ Estadual Londrina, CCE, Dept Quim, BR-86051970 Londrina, PR, Brazil
[2] Univ Estadual Londrina, CCB, Dept Ciencias Fisiol, BR-86051970 Londrina, PR, Brazil
[3] Univ Sao Paulo, Inst Quim, BR-05599970 Sao Paulo, Brazil
来源：
QUIMICA NOVA | 1998年 / 21卷 / 06期
关键词：
protein; spectrophotometry; protein analysis;
D O I：
10.1590/S0100-40421998000600020
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Spectrophotometric determination of total protein is used in several areas such as clinical analysis, food science and technology, biochemistry, protein chemistry, physiology. Five spectrophotometric methods are mostly used: biuret, Lowry, Bradford, Smith and UV absorption. In this review a general overview of these methods is presented (interferences, applications); other methodologies are also discussed.
引用
收藏
页码：787 / 793
页数：7
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