Purification and structure analysis of antifreeze proteins from ammopiptanthus mongolicus

被引：26

作者：

Fei, Y. B. ^{[2
]}

Cao, P. X. ^{[2
,3
]}

Gao, S. Q. ^{[2
]}

Wang, B. ^{[1
]}

Wei, L. B. ^{[2
]}

Zhao, J. ^{[2
]}

Chen, G. ^{[2
]}

Wang, B. H. ^{[1
]}

机构：

[1] Peking Univ, Inst Phys Chem, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2008年 / 38卷 / 02期

关键词：

Ammopiptanthus mongolicus; antifreeze proteins; circular dichroism spectroscopy; differential scanning calorimetry; inhibition of the growth of ice crystals; sequence analysis;

D O I：

10.1080/10826060701885126

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We purified many kinds of antifreeze proteins with high activity from the leaves of Ammopiptanthus mongolicus by several biochemical techniques. The antifreeze activities of these AFPs were measured by both osmometry and differential scanning calorimetry, and the inhibition of growth of ice crystals by the AFPs was obvious. Additionally, the antifreeze proteins were analyzed by sequencing, glycosylation reaction, mass spectroscopy, and circular dichroism spectroscopy. Both samples have some other unique structures different from those of fishes and of insects. It was suggested that plant AFPs might have a particular antifreeze mechanism in comparison with that of fish and insects.

引用

页码：179 / 190

页数：12

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