Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography

被引：55

作者：

Guo, Shuying ^{[1
]}

Feng, Bo ^{[1
]}

Zhu, Ruonan ^{[2
]}

Ma, Jiankang ^{[1
]}

Wang, Wei ^{[2
]}

机构：

[1] Jilin Med Coll, Coll Pharm, Changchun 132013, Jilin, Peoples R China

[2] Jilin Acad Chinese Med Sci, Inst Phytochem, Changchun 130012, Peoples R China

来源：

MOLECULES | 2011年 / 16卷 / 02期

关键词：

Rumex japonicus Houtt; anthraquinones; high-speed counter-current chromatography (HSCCC); emodin; chrysophanol; physcion; SEPARATION; PURIFICATION; RHEUM; HYDROXYANTHRAQUINONES; GLYCOSIDES; RHUBARB; L;

D O I：

10.3390/molecules16021201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three anthraquinones-emodin, chrysophanol, and physcion-were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane-ethanol-water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by H-1-NMR and C-13-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.

引用

页码：1201 / 1210

页数：10

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