Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping

被引：50

作者：

Guan, Jia ^{[1
]}

Zhao, Jing ^{[1
]}

Feng, Kun ^{[1
]}

Hu, De-Jun ^{[1
]}

Li, Shao-Ping ^{[1
]}

机构：

[1] Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 399卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Cordyceps; Polysaccharides; Enzymatic digestion; Saccharide mapping; High-performance size-exclusion chromatography; PERFORMANCE LIQUID-CHROMATOGRAPHY; TRADITIONAL CHINESE MEDICINE; CAPILLARY-ELECTROPHORESIS; MASS-SPECTROMETRY; 11; NUCLEOSIDES; SINENSIS; BASES; EXTRACTION; MYCELIA; HPLC;

D O I：

10.1007/s00216-010-4396-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison and characterization of polysaccharides from natural and cultured Cordyceps on the basis of their chemical characteristics such as glycosidic linkages were performed for the first time using saccharide mapping. The results showed that polysaccharides from most of the natural and cultured Cordyceps had similar responses to enzymatic digestion. These polysaccharides mainly contained (1 -> 4)-beta-D-glucosidic linkages, and (1 -> 4)-alpha-glucosidic, (1 -> 6)-alpha-glucosidic, 1,4-beta-D-mannosidic, as well as (1 -> 4)-alpha-D-galactosiduronic linkages also existed in some polysaccharides. Especially, natural and cultured Cordyceps polysaccharides could be discriminated on the basis of high performance liquid chromatography profiles of pectinase hydrolysates, which is helpful to control the quality of polysaccharides from Cordyceps.

引用

页码：3465 / 3474

页数：10

共 40 条

[1] BACTERIAL DEXTRANASE [J].

BAILEY, RW ;

CLARKE, RTJ .

BIOCHEMICAL JOURNAL, 1959, 72 :49-54

[2]

BARRAS DR, 1969, CELLULASES THEIR APP

[3] Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) [J].

Fan, H. ;

Li, S. P. ;

Xiang, J. J. ;

Lai, C. M. ;

Yang, F. Q. ;

Gao, J. L. ;

Wang, Y. T. .

ANALYTICA CHIMICA ACTA, 2006, 567 (02) :218-228

[4] Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis [J].

Gong, YX ;

Li, SP ;

Li, P ;

Liu, JJ ;

Wang, YT .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1055 (1-2) :215-221

[5] Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping-Enzymatic digestion followed by chromatographic analysis [J].

Guan, J. ;

Li, S. P. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2010, 51 (03) :590-598

[6] Evaluation of Carbohydrates in Natural and Cultured Cordyceps by Pressurized Liquid Extraction and Gas Chromatography Coupled with Mass Spectrometry [J].

Guan, Jia ;

Yang, Feng-Qing ;

Li, Shao-Ping .

MOLECULES, 2010, 15 (06) :4227-4241

[7]

Larner J., 1960, ENZYMES

[8] Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris [J].

Lee, Jong Seok ;

Kwon, Jeong Seok ;

Yun, Jong Seok ;

Pahk, Jung Woon ;

Shin, Won Cheol ;

Lee, Shin Young ;

Hong, Eock Kee .

CARBOHYDRATE POLYMERS, 2010, 80 (04) :1011-1017

[9] Chemical properties and antioxidant activity of exopolysaccharides from mycelial culture of Cordyceps sinensis fungus Cs-HK1 [J].

Leung, Po Hong ;

Zhao, Shuna ;

Ho, Kwok Ping ;

Wu, Jian Yong .

FOOD CHEMISTRY, 2009, 114 (04) :1251-1256

[10] Quality control of Cordyceps sinensis, a valued traditional Chinese medicine [J].

Li, S. P. ;

Yang, F. Q. ;

Tsim, Karl W. K. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 41 (05) :1571-1584

← 1 2 3 4 →