Proteomic LC-MS systems using nanoscale liquid chromatography with tandem mass spectrometry

被引：134
作者：
Ishihama, Y [1 ]
机构：
[1] Eisai & Co Ltd, Lab Seeds Finding Technol, Tsukuba, Ibaraki 3002635, Japan
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1067卷 / 1-2期
关键词：
proteome; proteomics; NanoLC-MS/MS;
D O I：
10.1016/j.chroma.2004.10.107
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Current nano-scale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) approaches in proteome research are reviewed from an analytical perspective. For comprehensive analysis of cellular proteins, analytical methods with higher resolution, sensitivity, and wider dynamic range are required. Miniaturized LC coupled with tandem mass spectrometry is currently one of the most versatile techniques. In this review, the current status of nanoLC-MS/MS systems as well as data management systems is addressed. In addition, the future prospects for complete proteomics are discussed. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：73 / 83
页数：11
相关论文
共 111 条
  • [31] Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry (Reprinted from Science, vol 255, March 6, 1992)
    Hunt, Donald F.
    Henderson, Robert A.
    Shabanowitz, Jeffrey
    Sakaguchi, Kazuyasu
    Michel, Hanspeter
    Sevilir, Noelle
    Cox, Andrea L.
    Appella, Ettore
    Engelhard, Victor H.
    [J]. JOURNAL OF IMMUNOLOGY, 2007, 179 (05) : 2669 - 2671
  • [32] Highly robust stainless steel tips as micro electro spray emitters
    Ishihama, Y
    Katayama, H
    Asakawa, N
    Oda, Y
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (10) : 913 - 918
  • [33] Surfactants usable for electrospray ionization mass spectrometry
    Ishihama, Y
    Katayama, H
    Asakawa, N
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 287 (01) : 45 - 54
  • [34] ISHIHAMA Y, 2002, P 50 ASMS C ORL FL U, DOI UNSP THPA009
  • [35] ISHIHAMA Y, 2002, P 51 ASMS C MASS SPE, DOI UNSP MPX489
  • [36] ISHIHAMA Y, 2003, CHROMATOGRAPHY S1, V24, P12
  • [37] Performance of a monolithic silica column in a capillary under pressure-driven and electrodriven conditions
    Ishizuka, N
    Minakuchi, H
    Nakanishi, K
    Soga, N
    Nagayama, H
    Hosoya, K
    Tanaka, N
    [J]. ANALYTICAL CHEMISTRY, 2000, 72 (06) : 1275 - 1280
  • [38] DIRECT COUPLING OF MICRO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FAST ATOM BOMBARDMENT MASS-SPECTROMETRY
    ITO, Y
    TAKEUCHI, T
    ISHII, D
    [J]. JOURNAL OF CHROMATOGRAPHY, 1985, 346 (OCT): : 161 - 166
  • [39] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS
    KARAS, M
    HILLENKAMP, F
    [J]. ANALYTICAL CHEMISTRY, 1988, 60 (20) : 2299 - 2301
  • [40] SEPARATION EFFICIENCY OF SLURRY-PACKED LIQUID-CHROMATOGRAPHY MICROCOLUMNS WITH VERY SMALL INNER DIAMETERS
    KARLSSON, KE
    NOVOTNY, M
    [J]. ANALYTICAL CHEMISTRY, 1988, 60 (17) : 1662 - 1665
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