Characterization of a human cardiac gene which encodes for a LIM domain protein and is developmentally expressed in myocardial development

被引：14
作者：
Fung, YWW
Wang, RX
Liew, CC
机构：
[1] UNIV TORONTO, TORONTO HOSP, DEPT CLIN BIOCHEM, HUMAN CARDIAC GENE UNIT, TORONTO, ON M5G 1L5, CANADA
[2] UNIV TORONTO, TORONTO HOSP, CARDIOVASC RES CTR, TORONTO, ON M5G 1L5, CANADA
[3] UNIV TORONTO, TORONTO HOSP, DEPT MED, TORONTO, ON M5G 1L5, CANADA
来源：
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 1996年 / 28卷 / 06期
基金：
英国医学研究理事会;
关键词：
LIM protein; human heart cDNA library; zinc fingers;
D O I：
10.1006/jmcc.1996.0111
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A clone with high sequence homology to a rat muscle LIM protein has been isolated from a human fetal heart cDNA library. The isolated cDNA is 887 bp in length, with an open reading frame of 194 amino acid residues. Northern blot analysis revealed that it is predominantly expressed in fetal and neonatal hearts, with a low level of expression in adult heart and slow-twitch skeletal muscle (soleus). No detectable expression of this transcript was found in other tissues. Its cardiac expression and structural similarity to the rat homolog implied a regulatory role in human cardiac muscle. The biological property was confirmed by zinc binding assay, demonstrating its zinc-binding affinity. Homology of CLP to a rat muscle LIM protein and its predominantly differential expression in cardiac tissue imply its significant putative role in the development and growth of the human heart. (C) 1996 Academic Press Limited
引用
收藏
页码：1203 / 1210
页数：8
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