Molecular cloning of human thiamin pyrophosphokinase

被引：22
|
作者：
Zhao, RB
Gao, F
Goldman, ID
机构：
[1] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA
[2] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA
[3] Albert Einstein Coll Med, Albert Einstein Comprehens Canc Ctr, Bronx, NY 10461 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1517卷 / 02期
关键词：
thiamin; pyrophosphate; pyrophosphokinase; cofactor;
D O I：
10.1016/S0167-4781(00)00264-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：320 / 322
页数：3
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