Fungal Degradation of Extractives Plays an Important Role in the Brown Rot Decay of Scots Pine Heartwood

被引:6
作者
Belt, Tiina [1 ]
Harju, Anni [2 ]
Kilpelainen, Petri [3 ]
Venalainen, Martti [2 ]
机构
[1] Nat Resources Inst Finland, Prod Syst Unit, Biomass Characterizat & Properties, Espoo, Finland
[2] Nat Resources Inst Finland, Prod Syst Unit, Biomass Characterizat & Properties, Savonlinna, Finland
[3] Nat Resources Inst Finland, Prod Syst Unit, Biorefinery & Bioprod, Espoo, Finland
基金
芬兰科学院;
关键词
brown rot; Coniophora puteana; heartwood; hydroxylation; resin acid; Rhodonia placenta; stilbene; pinosylvin; CELLULAR-LEVEL DISTRIBUTIONS; LIPOPHILIC EXTRACTIVES; WOOD; LIGNIN; RESVERATROL; DURABILITY; RESISTANCE; REVEALS; COX-1; ACID;
D O I
10.3389/fpls.2022.912555
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Scots pine heartwood is known to have resistance to wood decay due to the presence of extractives, namely stilbenes and resin acids. However, previous studies have indicated that these extractives are degradable by wood decaying fungi. This study aimed to investigate the relationship between extractive degradation and heartwood decay in detail and to gain insight into the mechanisms of extractive degradation. Mass losses recorded after a stacked-sample decay test with brown rot fungi showed that the heartwood had substantial decay resistance against Coniophora puteana but little resistance against Rhodonia placenta. Extracts obtained from the decayed heartwood samples revealed extensive degradation of stilbenes by R. placenta in the early stages of decay and a noticeable but statistically insignificant loss of resin acids. The extracts from R. placenta-degraded samples contained new compounds derived from the degraded extractives: hydroxylated stilbene derivatives appeared in the early decay stages and then disappeared, while compounds tentatively identified as hydroxylated derivatives of dehydroabietic acid accumulated in the later stages. The degradation of extractives was further analysed using simple degradation assays where an extract obtained from intact heartwood was incubated with fungal mycelium or extracellular culture fluid from liquid fungal cultures or with neat Fenton reagent. The assays showed that extractives can be eliminated by several fungal degradative systems and revealed differences between the degradative abilities of the two fungi. The results of the study indicate that extractive degradation plays an important role in heartwood decay and highlight the complexity of the fungal degradative systems.
引用
收藏
页数:11
相关论文
共 41 条
[1]  
[Anonymous], 2016, EN 350
[2]   Peculiarities of brown-rot fungi and biochemical Fenton reaction with regard to their potential as a model for bioprocessing biomass [J].
Arantes, Valdeir ;
Jellison, Jody ;
Goodell, Barry .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 94 (02) :323-338
[3]   Extractive concentrations and cellular-level distributions change radially from outer to inner heartwood in Scots pine [J].
Belt, Tiina ;
Venalainen, Martti ;
Altgen, Michael ;
Harju, Anni ;
Rautkari, Lauri .
TREE PHYSIOLOGY, 2021, 41 (06) :1034-1045
[4]   Non-destructive measurement of Scots pine heartwood stilbene content and decay resistance by means of UV-excited fluorescence spectroscopy [J].
Belt, Tiina ;
Venalainen, Martti ;
Harju, Anni .
INDUSTRIAL CROPS AND PRODUCTS, 2021, 164
[5]   Cellular level chemical changes in Scots pine heartwood during incipient brown rot decay [J].
Belt, Tiina ;
Altgen, Michael ;
Makela, Mikko ;
Hanninen, Tuomas ;
Rautkari, Lauri .
SCIENTIFIC REPORTS, 2019, 9 (1)
[6]   Cellular level distributions of Scots pine heartwood and knot heartwood extractives revealed by Raman spectroscopy imaging [J].
Belt, Tiina ;
Keplinger, Tobias ;
Hanninen, Tuomas ;
Rautkari, Lauri .
INDUSTRIAL CROPS AND PRODUCTS, 2017, 108 :327-335
[7]   Modelling the Material Resistance of Wood-Part 3: Relative Resistance in above- and in-Ground Situations-Results of a Global Survey [J].
Brischke, Christian ;
Alfredsen, Gry ;
Humar, Miha ;
Conti, Elena ;
Cookson, Laurie ;
Emmerich, Lukas ;
Flaete, Per Otto ;
Fortino, Stefania ;
Francis, Lesley ;
Hundhausen, Ulrich ;
Irbe, Ilze ;
Jacobs, Kordula ;
Klamer, Morten ;
Krzisnik, Davor ;
Lesar, Bostjan ;
Melcher, Eckhard ;
Meyer-Veltrup, Linda ;
Morrell, Jeffrey J. ;
Norton, Jack ;
Palanti, Sabrina ;
Presley, Gerald ;
Reinprecht, Ladislav ;
Singh, Tripti ;
Stirling, Rod ;
Venalainen, Martti ;
Westin, Mats ;
Wong, Andrew H. H. ;
Suttie, Ed .
FORESTS, 2021, 12 (05)
[8]   Extractive distribution in Pseudotsuga menziesii: effects on cell wall porosity in sapwood and heartwood [J].
Donaldson, Lloyd A. ;
Singh, Adya ;
Raymond, Laura ;
Hill, Stefan ;
Schmitt, Uwe .
IAWA JOURNAL, 2019, 40 (04) :721-740
[9]   Versatile biocatalysis of fungal cytochrome P450 monooxygenases [J].
Durairaj, Pradeepraj ;
Hur, Jae-Seoun ;
Yun, Hyungdon .
MICROBIAL CELL FACTORIES, 2016, 15
[10]   Heartwood extractives and lignin content of different larch species (Larix sp.) and relationships to brown-rot decay-resistance [J].
Gierlinger, N ;
Jacques, D ;
Schwanninger, M ;
Wimmer, R ;
Pâques, LE .
TREES-STRUCTURE AND FUNCTION, 2004, 18 (02) :230-236