Minimally Invasive Micro-Indentation: mapping tissue mechanics at the tip of an 18G needle

被引:15
作者
Beekmans, Steven V. [1 ,2 ]
Emanuel, Kaj S. [3 ]
Smit, Theodoor H. [4 ,5 ]
Iannuzzi, Davide [1 ,2 ]
机构
[1] Vrije Univ Amsterdam, Dept Phys & Astron, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, LaserLab Amsterdam, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands
[3] Vrije Univ Amsterdam, Med Ctr VUmc, Dept Orthopaed Surg, Amsterdam Movement Sci, De Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands
[4] AMC, Dept Med Biol, Meiberdreef 9, NL-1105 AZ Amsterdam, Netherlands
[5] AMC, Dept Orthoped Surg, Meiberdreef 9, NL-1105 AZ Amsterdam, Netherlands
基金
欧洲研究理事会;
关键词
NUCLEUS PULPOSUS; INTERVERTEBRAL DISC; RHEOLOGICAL CHARACTERIZATION; EXTRACELLULAR-MATRIX; SOFT-TISSUE; CARTILAGE; DEGENERATION; ELASTOGRAPHY; SCAFFOLDS; BEHAVIOR;
D O I
10.1038/s41598-017-10526-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Experiments regarding the mechanical properties of soft tissues mostly rely on data collected on specimens that are extracted from their native environment. During the extraction and in the time period between the extraction and the completion of the measurements, however, the specimen may undergo structural changes which could generate unwanted artifacts. To further investigate the role of mechanics in physiology and possibly use it in clinical practices, it is thus of paramount importance to develop instruments that could measure the viscoelastic response of a tissue without necessarily excising it. Tantalized by this opportunity, we have designed a minimally invasive micro-indenter that is able to probe the mechanical response of soft tissues, in situ, via an 18G needle. Here, we discuss its working principle and validate its usability by mapping the viscoelastic properties of a complex, confined sample, namely, the nucleus pulposus of the intervertebral disc. Our findings show that the mechanical properties of a biological tissue in its local environment may be indeed different than those that one would measure after excision, and thus confirm that, to better understand the role of mechanics in life sciences, one should always perform minimally invasive measurements like those that we have here introduced.
引用
收藏
页数:8
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