Quantification of Tricyclic and Nontricyclic Antidepressants in Spiked Plasma and Urine Samples Using Microextraction in Packed Syringe and Analysis by LC and GC-MS

被引:34
作者
Rani, Susheela [1 ]
Kumar, Ashwini [1 ]
Malik, Ashok Kumar [1 ]
Singh, Baldev [1 ]
机构
[1] Punjabi Univ, Dept Chem, Patiala 147002, Punjab, India
关键词
HPLC; Gas chromatography-mass spectrometry; Microextraction in packed syringes; Antidepressants; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; SEROTONIN REUPTAKE INHIBITORS; SOLID-PHASE EXTRACTION; QUANTITATIVE-ANALYSIS; ACTIVE METABOLITES; WHOLE-BLOOD; ONLINE; SORBENT; MEPS;
D O I
10.1007/s10337-011-2052-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple, fast, selective and very sensitive method based on sample preparation by the relatively new technique of microextraction in a packed syringe (MEPS) and analysis by liquid chromatography (LC) and gas chromatography-mass spectrometry (GC-MS) for the simultaneous determination of antidepressant drugs (amitriptyline, imipramine, clomipramine, mirtazapine and citalopram) was developed. MEPS is a new miniaturized, solid-phase extraction (SPE) technique that can be connected to LC or GC without any modifications. It is very easy to use, fully automated, inexpensive, quick and uses very small amounts (in mu L) of organic solvents. Drugs are extracted using the MEPS technique followed by their analysis using LC-UV and GC-MS. Limits of detection were in the range of 0.088-0.284 ng mL(-1) on GC-MS and between 0.133 and 0.360 ng mL(-1) on LC-UV. The purpose of this study is to develop and validate MEPS as a new sample preparation technique for antidepressant drugs using chromatographic methods to detect these drugs in human plasma and urine samples.
引用
收藏
页码:235 / 242
页数:8
相关论文
共 42 条
[1]   Microextraction in packed syringe (MEPS) for liquid and gas chromatographic applications. Part II - Determination of ropivacaine and its metabolites in human plasma samples using MEPS with liquid chromatography/tandem mass spectrometry [J].
Abdel-Rehim, M ;
Altun, Z ;
Blomberg, L .
JOURNAL OF MASS SPECTROMETRY, 2004, 39 (12) :1488-1493
[2]   New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications - I. Determination of local anaesthetics in human plasma samples using gas chromatography-mass spectrometry [J].
Abdel-Rehim, M .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 801 (02) :317-321
[3]   Quantification of ropivacaine and its major metabolites in human urine samples utilizing microextraction in a packed syringe automated with liquid chromatography-tandem mass spectrometry (MEPS-LC-MS/MS) [J].
Abdel-Rehim, Mohamed ;
Dahlgren, Marie ;
Blomberg, Lars .
JOURNAL OF SEPARATION SCIENCE, 2006, 29 (11) :1658-1661
[4]   Drug screening using microextraction in a packed syringe (MEPS)/mass spectrometry utilizing monolithic-, polymer-, and silica-based sorbents [J].
Altun, Z ;
Blomberg, LG ;
Jagerdeo, E ;
Abdel-Rehim, M .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2006, 29 (06) :829-840
[5]   New trends in sample preparation: on-line microextraction in packed syringe (MEPS) for LC and GC applications Part III: Determination and validation of local anaesthetics in human plasma samples using a cation-exchange sorbent, and MEPS-LC-MS-MS [J].
Altun, Z ;
Abdel-Rehim, M ;
Blomberg, LG .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 813 (1-2) :129-135
[6]   Study of the factors affecting the performance of microextraction by packed sorbent (MEPS) using liquid scintillation counter and liquid chromatography-tandem mass spectrometry [J].
Altun, Zeki ;
Abdel-Rehim, Mohamed .
ANALYTICA CHIMICA ACTA, 2008, 630 (02) :116-123
[7]   Optimization of the SPME parameters and its online coupling with HPLC for the analysis of tricyclic antidepressants in plasma samples [J].
Alves, Claudete ;
Fernandes, Christian ;
Santos-Neto, Alvaro Jose ;
Rodrigues, Jose Carlos ;
Costa Queiroz, Maria Eugenia ;
Lancas, Fernando Mauro .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2006, 44 (06) :340-346
[8]  
Askemark Y., 2006, J LIQ CHROMATOGR R T, V29, P2413
[9]   Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection [J].
Chaves, Andrea R. ;
Leandro, Fernanda Z. ;
Carris, Juciene A. ;
Queiroz, Maria Eugenia C. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (23) :2123-2129
[10]  
DURVERNEUIL C, 2003, THER DRUG MONIT, V25, P565