EMBRYONIC STEM CELLS IMPROVE SKELETAL MUSCLE RECOVERY AFTER EXTREME ATROPHY IN MICE

被引：4

作者：

Giannini, Guilherme ^{[1
,2
]}

De Oliveira Silvestre, Joao Guilherme ^{[1
]}

Limongi Franca Guilherme, Joao Paulo ^{[2
]}

Baptista, Igor Luchini ^{[1
]}

Ramos, Gracielle Vieira ^{[1
]}

Da Silva, Willian Jose ^{[1
]}

Miyabara, Elen Haruka ^{[3
]}

Moriscot, Anselmo Sigari ^{[1
]}

机构：

[1] Inst Biomed Sci, Dept Anat, Lab Cellular & Mol Biol Striated Muscle, BR-05508000 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Biodynam, Sch Phys Educ & Sport, Lab Appl Nutr & Metab, Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Skeletal Muscle Plast, Sao Paulo, Brazil

来源：

MUSCLE & NERVE | 2015年 / 51卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

atrophy; botulinum toxin; contractile function; embryonic stem cells; skeletal muscle; RESISTANCE EXERCISE; MUSCULAR-DYSTROPHY; TRANSPLANTATION; CARDIOMYOCYTES; HYPERTROPHY; GENERATION; MYOBLASTS;

D O I：

10.1002/mus.24320

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

IntroductionWe injected embryonic stem cells into mouse tibialis anterior muscles subjected to botulinum toxin injections as a model for reversible neurogenic atrophy. MethodsMuscles were exposed to botulinum toxin for 4 weeks and allowed to recover for up to 6 weeks. At the onset of recovery, a single muscle injection of embryonic stem cells was administered. The myofiber cross-sectional area, single twitch force, peak tetanic force, time-to-peak force, and half-relaxation time were determined. ResultsAlthough the stem cell injection did not affect the myofiber cross-sectional area gain in recovering muscles, most functional parameters improved significantly compared with those of recovering muscles that did not receive the stem cell injection. ConclusionsMuscle function recovery was accelerated by embryonic stem cell delivery in this durable neurogenic atrophy model. We conclude that stem cells should be considered a potential therapeutic tool for recovery after extreme skeletal muscle atrophy. Muscle Nerve 51: 346-352, 2015

引用

页码：346 / 352

页数：7

共 27 条

[21] Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors [J].