The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway

被引：70

作者：

Basbous, Nada ^{[2
]}

Coste, Franck ^{[2
]}

Leone, Philippe ^{[1
,3
]}

Vincentelli, Renaud ^{[1
,3
]}

Royet, Julien ^{[3
,4
]}

Kellenberger, Christine ^{[2
]}

Roussel, Alain ^{[1
,2
]}

机构：

[1] CNRS, UMR6098, F-13288 Marseille 9, France

[2] CNRS, UPR 4301, Ctr Biophys Mol, F-45071 Orleans, France

[3] Aix Marseille Univ, F-13288 Marseille 9, France

[4] CNRS, UMR6216, Inst Biol Dev Marseille Luminy, F-13288 Marseille 9, France

来源：

EMBO REPORTS | 2011年 / 12卷 / 04期

关键词：

Drosophila; innate immunity; structural biology; IMMUNE-RESPONSE; PGRP-LC; BACTERIA;

D O I：

10.1038/embor.2011.19

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The peptidoglycan (PGN)-recognition protein LF (PGRP-LF) is a specific negative regulator of the immune deficiency (Imd) pathway in Drosophila. We determine the crystal structure of the two PGRP domains constituting the ectodomain of PGRP-LF at 1.72 and 1.94 angstrom resolution. The structures show that the LFz and LFw domains do not have a PGN-docking groove that is found in other PGRP domains, and they cannot directly interact with PGN, as confirmed by biochemical-binding assays. By using surface plasmon resonance analysis, we show that the PGRP-LF ectodomain interacts with the PGRP-LCx ectodomain in the absence and presence of tracheal cytotoxin. Our results suggest a mechanism for downregulation of the Imd pathway on the basis of the competition between PRGP-LCa and PGRP-LF to bind to PGRP-LCx.

引用

收藏

页码：327 / 333

页数：7

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