Effects of oxymatrine on sympathoexcitatory reflex induced by myocardial ischemic signaling mediated by P2X3 receptors in rat SCG and DRG

被引：45
|
作者：
Li, Guilin [1 ]
Liu, Shuangmei [1 ]
Yang, Yang [2 ]
Xie, Jinyan [1 ]
Liu, Jun [1 ]
Kong, Fanjun [1 ]
Tu, Guihua [1 ]
Wu, Raoping [1 ]
Li, Guodong [1 ]
Liang, Shangdong [1 ]
机构：
[1] Nanchang Univ, Coll Med, Dept Physiol, Nanchang 330006, Jiangxi, Peoples R China
[2] Nanchang Univ, Coll Med, Dept Clin Med 1, Nanchang 330006, Jiangxi, Peoples R China
来源：
BRAIN RESEARCH BULLETIN | 2011年 / 84卷 / 06期
基金：
中国国家自然科学基金;
关键词：
P2X(3) receptor; Oxymatrine; SCG; DRG; Myocardial ischemic injury; CARDIAC SYMPATHETIC AFFERENTS; P2X(2/3) RECEPTOR; P2; RECEPTORS; ADENOSINE 5'-TRIPHOSPHATE; GANGLION NEURONS; PAIN; ATP; RELEASE; INVOLVEMENT; ACTIVATION;
D O I：
10.1016/j.brainresbull.2011.01.011
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Sympathoexcitatory reflex is characterized by an increase in blood pressure and sympathetic nerve activity. P2X(3) receptors in SCG neurons are involved in increasing sympathoexcitatory reflex after myocardial ischemic (MI) injury. The present study is aimed to explore the effects of oxymatrine (Oxy) on the transmission of myocardial ischemic signaling mediated by P2X(3) receptors in rat superior cervical ganglia (SCG) and cervical dorsal root ganglia (DRG) in the sympathoexcitatory reflex after myocardial ischemic injury. In this study, the expression levels of P2X(3) immunoreactivity, mRNA and protein were analyzed in SCG and DRG neurons by immunohistochemistry, in situ hybridization and Western blotting. The results show that the myocardial ischemic injury induce:; the increase of the systolic blood pressure and heart rate and upregulates the expression of P2X(3) receptors in SCG and DRG neurons. Upregulated expression of P2X(3) receptors in SCG and DRG neurons subsequently leads to the aggravated sympathoexcitatory reflex. Oxymatrine reduces the systolic blood pressure and heart rate in myocardial ischemic rats. After myocardial ischemic rats are treated with oxymatrine, the expression levels of P2X(3) immunoreactivity, mRNA and protein are lower than those in myocardial ischemic rats. Oxymatrine may decrease the expression of P2X(3) receptor and depress the aggravated sympathoexcitatory reflex induced by the nociceptive transmission of myocardial ischemic injury via P2X(3) receptors of rat SCG and DRG neurons. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：419 / 424
页数：6
相关论文
共 50 条
  • [21] Inhibition of urothelial P2X3 receptors prevents desensitization of purinergic detrusor contractions in the rat bladder
    Ferguson, Andrew C.
    Sutton, Broderick W.
    Boone, Timothy B.
    Ford, Anthony P.
    Munoz, Alvaro
    BJU INTERNATIONAL, 2015, 116 (02) : 293 - 301
  • [22] Estrogen altered visceromotor reflex and P2X3 mRNA expression in a rat model of colitis
    Fan, Juan
    Yu, Li-hua
    Zhang, Yanmin
    Ni, Xin
    Ma, Bei
    Burnstock, Geoffrey
    STEROIDS, 2009, 74 (12) : 956 - 962
  • [23] 2 Hz EA Reduces Heroin Withdrawal-Induced Hyperalgesia and Heroin Relapse by Downregulating P2X3 Receptors in DRG Neurons
    Chen, Lin
    Leng, Changlong
    Gong, Xiaokang
    Ma, Baomiao
    Ru, Qin
    Xiong, Qi
    Zhou, Mei
    Tian, Xiang
    Yue, Kai
    Li, Chaoying
    Wu, Yuxiang
    BIOMED RESEARCH INTERNATIONAL, 2019, 2019
  • [24] Upregulation of P2X2 and P2X3 receptors in rats with hyperalgesia induced by heroin withdrawal
    Leng, Changlong
    Chen, Lin
    Gong, Xiaokang
    Ma, Baomiao
    Gan, Weimin
    Si, Yuanren
    Xiao, Huaqiao
    Li, Chaoying
    NEUROREPORT, 2018, 29 (08) : 678 - 684
  • [25] Molecular mechanism for opioid dichotomy: bidirectional effect of μ-opioid receptors on P2X3 receptor currents in rat sensory neurones
    Chizhmakov, Igor
    Kulyk, Vyacheslav
    Khasabova, Iryna
    Khasabov, Sergey
    Simone, Donald
    Bakalkin, Georgy
    Gordienko, Dmitri
    Verkhratsky, Alexei
    Krishtal, Oleg
    PURINERGIC SIGNALLING, 2015, 11 (02) : 171 - 181
  • [26] The involvement of P2X3 receptors of rat sympathetic ganglia in cardiac nociceptive transmission
    Zhang, C. P.
    Xu, C. S.
    Liang, S. D.
    Li, G. L.
    Gao, Y.
    Wang, Y. X.
    Zhang, A. X.
    Wan, F.
    JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY, 2007, 63 (03) : 249 - 257
  • [27] Identification of P2X3 and P2X7 Purinergic Receptors Activated by ATP in Rat Lacrimal Gland
    Hodges, Robin R.
    Vrouvlianis, Joanna
    Scott, Rachel
    Dartt, Darlene A.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2011, 52 (06) : 3254 - 3263
  • [28] Puerarin alleviates burn-related procedural pain mediated by P2X3 receptors
    Xin Li
    Jun Zhang
    Yun Gao
    Yang Yang
    Changshui Xu
    Guilin Li
    Guanghua Guo
    Shuangmei Liu
    Jinyan Xie
    Shangdong Liang
    Purinergic Signalling, 2011, 7 : 489 - 497
  • [29] Extracellular cAMP inhibits P2X3 receptors in rat sensory neurones through G protein-mediated mechanism
    Mamenko, M. V.
    Chizhmakov, I. V.
    Volkova, T. M.
    Verkhratsky, A.
    Krishtal, O. A.
    ACTA PHYSIOLOGICA, 2010, 199 (02) : 199 - 204
  • [30] Enhancement of P2X3 Receptor-Mediated Currents by Lysophosphatidic Acid in Rat Primary Sensory Neurons
    Qiao, Wen-Long
    Li, Qing
    Hao, Jia-Wei
    Wei, Shuang
    Li, Xue-Mei
    Liu, Ting-Ting
    Qiu, Chun-Yu
    Hu, Wang-Ping
    FRONTIERS IN PHARMACOLOGY, 2022, 13
12345