Analysis of the marker compounds of Rhodiola rosea L. (golden root) by reversed phase high performance liquid chromatography

被引：83
作者：
Ganzera, M
Yayla, Y
Khan, IA [1 ]
机构：
[1] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA
[2] Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornava Izmir, Turkey
[3] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 2001年 / 49卷 / 04期
关键词：
Rhodiola rosea; HPLC; phenylpropanoid; salidroside; rosavin;
D O I：
10.1248/cpb.49.465
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
An HPLC method permitting the first simultaneous detection of 5 marker compounds (salidroside, rosarin, rosavin, rosin, rosiridin) of R, rosea was developed, A separation was achieved within 27 min by using C-18 column material, a phosphate buffer/acetonitrile gradient system and at a separation temperature of 60 degreesC. All five compounds could be detected at concentrations as low as 0.62 mug/ml and were clearly assignable in R. rosea plant material and commercial products. Therefore, this quantitative and qualitative applicability of the method offers efficient and reliable means for the evaluation of R. rosea and products thereof.
引用
收藏
页码：465 / 467
页数：3
相关论文
共 13 条
  • [1] BAO W, 1995, SHENYANG YAOKE DAXUE, V12, P263
  • [2] Rhodiola rosea in vitro culture - Phytochemical analysis and antioxidant action
    Furmanowa, M
    Skopinska-Rozewska, E
    Rogala, E
    Hartwich, M
    [J]. ACTA SOCIETATIS BOTANICORUM POLONIAE, 1998, 67 (01) : 69 - 73
  • [3] Rosavin as a product of glycosylation by Rhodiola rosea (roseroot) cell cultures
    Furmanowa, M
    Hartwich, M
    Alfermann, AW
    Kozminski, W
    Olejnik, M
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1999, 56 (02) : 105 - 110
  • [4] GANZERA M, IN PRESS PLANTA MED
  • [5] Germano C., 1999, Arctic Root (Rhodiola rosea): The Powerful New Ginseng Alternative
  • [6] KURKIN V A, 1986, Rastitel'nye Resursy, V22, P310
  • [7] Maslova L V, 1994, Eksp Klin Farmakol, V57, P61
  • [8] SARATIKOV AS, 1987, TOMSK, P252
  • [9] SOKOLOV SY, 1985, KHIM FARM ZH+, V19, P1367
  • [10] SOKOLOV SY, 1990, KHIM FARM ZH+, V24, P66
12