Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90

被引：4
|
作者：
Yamasaki, Masahiro [1 ]
Tsuboi, Yoshihiro [2 ]
Taniyama, Yusuke [2 ]
Uchida, Naohiro [1 ]
Sato, Reeko [1 ]
Nakamura, Kensuke [3 ]
Ohta, Hiroshi [2 ]
Takiguchi, Mitsuyoshi [2 ]
机构：
[1] Iwate Univ, Fac Agr, Dept Vet Med, Lab Vet Small Anim Internal Med, 3-18-8 Ueda, Morioka, Iwate 0208550, Japan
[2] Hokkaido Univ, Grad Sch Vet Med, Dept Vet Clin Sci, Lab Vet Internal Med, Sapporo, Hokkaido 0600818, Japan
[3] Hokkaido Univ, Grad Sch Vet Med, Vet Teaching Hosp, Sapporo, Hokkaido 0600818, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2016年 / 78卷 / 08期
关键词：
Babesia gibsoni; heat shock protein 90; phylogenetic analysis; STAGE DIFFERENTIATION; IN-VITRO; HSP90; HEAT-SHOCK-PROTEIN-90; SEQUENCE; GROWTH; IDENTIFICATION; EXPRESSION; PATHWAYS; ISOFORMS;
D O I：
10.1292/jvms.16-0027
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43 degrees C for 1 hr.
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收藏
页码：1355 / 1360
页数：6
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