Role of axl in preeclamptic EPCs functions

被引：5

作者：

Hu, Ying ^{[1
]}

Liu, Xiao-ping ^{[1
]}

Liu, Xiao-xia ^{[1
]}

Zheng, Yan-fang ^{[1
]}

Liu, Wei-fang ^{[1
]}

Luo, Ming-lian ^{[1
]}

Gao, Hui ^{[1
]}

Zhao, Ying ^{[1
]}

Zou, Li ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Union Hosp, Dept Obstet & Gynecol, Tongji Med Coll, Wuhan 430022, Peoples R China

来源：

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2016年 / 36卷 / 03期

关键词：

Axl; endothelial progenitor cells; preeclampsia; ENDOTHELIAL PROGENITOR CELLS; RECEPTOR TYROSINE KINASE; GAS6; SURVIVAL; PATHOGENESIS; DYSFUNCTION; EXPRESSION; PREGNANCY; PATHWAYS; GROWTH;

D O I：

10.1007/s11596-016-1598-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P < 0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.

引用

页码：395 / 401

页数：7

共 36 条

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