Identification of a murine cysteinyl leukotriene receptor by expression in Xenopus laevis oocytes

被引：15
|
作者：
Mollerup, J
Jorgensen, ST
Hougaard, C
Hoffmann, EK
机构：
[1] August Krogh Inst, Dept Biochem, DK-2100 Copenhagen O, Denmark
[2] Inst Mol Biol, Dept Mol Cell Biol, DK-1353 Copenhagen, Denmark
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1517卷 / 03期
关键词：
serpentine receptor; leukotriene; pertussis toxin; Xenopus laevis oocyte; MK-571;
D O I：
10.1016/S0167-4781(00)00271-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the identification of an EST encoding a murine cysteinyl leukotriene (mCysLT) receptor. LTD4, LTC4 and LTE4 but not LTB4 or various nucleotides activated Ca2+-evoked Cl- currents in mCysLT(1) expressing Xenopus laevis oocytes. The response to LTD4 was blocked by MK-571, reduced by pretreatment with pertussis toxin (PTX), and was partly dependent on extracellular Ca2+. The identified murine CysLT(1) receptor differs from the hCysLT(1) receptor with regard to PTX sensitivity, receptor-mediated Ca2+ influx, and antagonist sensitivity. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：455 / 459
页数：5
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