Longitudinal study of Staphylococcus aureus colonization and infection in a cohort of swine veterinarians in the United States

被引:19
作者
Sun, Jisun [1 ]
Yang, My [1 ]
Sreevatsan, Srinand [1 ]
Bender, Jeffrey B. [1 ]
Singer, Randall S. [2 ]
Knutson, Todd P. [1 ]
Marthaler, Douglas G. [1 ]
Davies, Peter R. [1 ]
机构
[1] Univ Minnesota, Dept Vet Populat Med, 385 ASVM,1988 Fitch Ave, St Paul, MN 55108 USA
[2] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
来源
BMC INFECTIOUS DISEASES | 2017年 / 17卷
关键词
Veterinarians; MRSA; Swine; Persistent carriage; PERSISTENT NASAL CARRIERS; METHICILLIN-RESISTANT; PIG FARMERS; CARRIAGE; PREVALENCE; MRSA; TRANSMISSION; DYNAMICS; STRAINS; HUMANS;
D O I
10.1186/s12879-017-2802-1
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: People working with pigs are at elevated risk of harboring methicillin resistant S. aureus (MRSA) in their nose, which is attributable to occupational exposure to animals harboring livestock adapted S. aureus. To obtain insight into the biological nature of occupationally related nasal culture positivity, we conducted a longitudinal study of 66 swine veterinarians in the USA. Methods: The study cohort resided in 15 US states and worked predominantly with swine. Monthly for 18 months, participants self-collected nasal swabs and completed a survey to report recent exposure to pigs and other animals; the occurrence of work related injuries; and any relevant health events such as skin and soft tissue infections or confirmed staphylococcal infections. Nasal swabs were cultured using selective methods to determine the presence of MRSA and methicillin susceptible S. aureus (MSSA), and isolates were characterized by spa typing and MLST. Results: Prevalences of S. aureus (64%, monthly range from 58 to 82%) and MRSA (9.5%; monthly range from 6 to15%) were higher than reported for the US population (30% and 1.5% respectively). Predominant spa types were t034 (ST398, 37%), t002 (ST5, 17%) and t337 (ST9/ST398 13%), a distribution similar to that found in a concurrent study in pigs in the USA. Veterinarians were classified into three groups: Persistent carriers (PC, 52%), Intermittent carriers (IC, 47%) and Non-carriers (NC, 1%). Persistent carriage of a single spa type was observed in 14 (21%) of participants, and paired (first and last) isolates from PC subjects had minor genetic differences. Swabs from PC veterinarians carried higher numbers of S. aureus. Among IC veterinarians, culture positivity was significantly associated with recent contact with pigs. Conclusions: Exposure to pigs did not lead to prolonged colonization in most subjects, and the higher numbers of S. aureus in PC subjects suggests that unknown host factors may determine the likelihood of prolonged colonization by S. aureus of livestock origin. Exposure to S. aureus and persistent colonization of swine veterinarians was common but rarely associated with S. aureus disease.
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页数:13
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共 61 条
[1]   Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans [J].
Alba, Patricia ;
Feltrin, Fabiola ;
Cordaro, Gessica ;
Concepcion Porrero, Maria ;
Kraushaar, Britta ;
Argudin, Maria Angeles ;
Nykasenoja, Suvi ;
Monaco, Monica ;
Stegger, Marc ;
Aarestrup, Frank M. ;
Butaye, Patrick ;
Franco, Alessia ;
Battisti, Antonio .
PLOS ONE, 2015, 10 (08)
[2]   Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs [J].
Armand-Lefevre, L ;
Ruimy, R ;
Andremont, A .
EMERGING INFECTIOUS DISEASES, 2005, 11 (05) :711-714
[3]   MRSA-ST398 in livestock farmers and neighbouring residents in a rural area in Germany [J].
Bisdorff, B. ;
Scholhoelter, J. L. ;
Claussen, K. ;
Pulz, M. ;
Nowak, D. ;
Radon, K. .
EPIDEMIOLOGY AND INFECTION, 2012, 140 (10) :1800-1808
[4]   Transmission through air as a possible route of exposure for MRSA [J].
Bos, Marian E. H. ;
Verstappen, Koen M. ;
van Cleef, Brigitte A. G. L. ;
Dohmen, Wietske ;
Dorado-Garcia, Alejandro ;
Graveland, Haitske ;
Duim, Birgitta ;
Wagenaar, Jaap A. ;
Kluytmans, Jan A. J. W. ;
Heederik, Dick J. J. .
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY, 2016, 26 (03) :263-269
[5]   Transmission and Persistence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus among Veterinarians and Their Household Members [J].
Bosch, T. ;
Verkade, E. ;
van Luit, M. ;
Landman, F. ;
Kluytmans, J. ;
Schouls, L. M. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2015, 81 (01) :124-129
[6]   Livestock-associated meticillin-resistant Staphylococcus aureus in Asia: An emerging issue? [J].
Chuang, Yu-Yu ;
Huang, Yhu-Chering .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2015, 45 (04) :334-340
[7]   Prevalence of the immune evasion gene cluster in Staphylococcus aureus CC398 [J].
Cuny, Christiane ;
Abdelbary, Mohamed ;
Layer, Franziska ;
Werner, Guido ;
Witte, Wolfgang .
VETERINARY MICROBIOLOGY, 2015, 177 (1-2) :219-223
[8]   Nasal Colonization of Humans with Methicillin-Resistant Staphylococcus aureus (MRSA) CC398 with and without Exposure to Pigs [J].
Cuny, Christiane ;
Nathaus, Rolf ;
Layer, Franziska ;
Strommenger, Birgit ;
Altmann, Doris ;
Witte, Wolfgang .
PLOS ONE, 2009, 4 (08)
[9]   Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study [J].
den Heijer, Casper D. J. ;
van Bijnen, Evelien M. E. ;
Paget, W. John ;
Pringle, Mike ;
Goossens, Herman ;
Bruggeman, Cathrien A. ;
Schellevis, Francois G. ;
Stobberingh, Ellen E. .
LANCET INFECTIOUS DISEASES, 2013, 13 (05) :409-415
[10]   Ten years later: still a high prevalence of MRSA in slaughter pigs despite a significant reduction in antimicrobial usage in pigs the Netherlands [J].
Dierikx, Cindy M. ;
Hengeveld, Paul D. ;
Veldman, Kees T. ;
de Haan, Angela ;
van der Voorde, Sanne ;
Dop, Petra Y. ;
Bosch, Thijs ;
van Duijkeren, Engeline .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2016, 71 (09) :2414-2418