Effects of Colored and Noncolored Phenolics of Echium plantagineum L. Bee Pollen in Caco-2 Cells under Oxidative Stress Induced by tert-Butyl Hydroperoxide

被引：25

作者：

Sousa, Carla ^{[1
]}

Moita, Eduarda ^{[1
]}

Valentao, Patricia ^{[1
]}

Fernandes, Fatima ^{[1
]}

Monteiro, Pedro ^{[1
]}

Andrade, Paula B. ^{[1
]}

机构：

[1] Univ Porto, Fac Farm, Dept Quim, REQUIMTE,LAQV,Lab Farmacognosia, P-4050313 Oporto, Portugal

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 07期

关键词：

Echium plantagineum L; bee pollen; Caco-2; cells; tert-butyl hydroperoxide; oxidative stress; RAT-LIVER; MITOCHONDRIAL-MEMBRANE; DRUG-METABOLISM; GLUTATHIONE; FLAVONOIDS; ANTHOCYANINS; INHIBITION; ALKALOIDS; TOXICITY; DAMAGE;

D O I：

10.1021/jf505568h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Bee pollen is used as a dietary supplement, being promoted as a health food. Echium plantagineum L. bee pollen fractions enriched in flavonols (fraction I) or anthocyanins (fraction II) and the whole extract were characterized by HPLC-DAD. Both in the whole extract and in fraction II seven flavonols and five anthocyanins were identified, while fraction I contained six flavonols (in higher levels than fraction II) and small amounts of petunidin-3-O-rutinoside. Antioxidant capacity was evaluated in Caco-2 cells under oxidative stress induced by tert-butyl hydroperoxide (t-BHP). Fraction I pre-exposure imparted a tendency to protect cells, while fraction II and the whole extract aggravated t-BHP toxicity at some concentrations. The protective effects seem to be correlated with the levels of total glutathione, while no correlation between cellular viability and reactive species was seen. The extracts displayed no significant effect on antioxidant enzymes activity. Overall, anthocyanins seem to abrogate the antioxidant potential of flavonoid-rich extracts.

引用

页码：2083 / 2091

页数：9

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