CRYOPRESERVED MESENCHYMAL STEM CELLS IMPACT ON THE SPINAL CORD TISSUE IN RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

被引：0
|
作者：
Semenova, V. M. [1 ]
Tsymbalyuk, V., I [1 ]
Liubich, L. D. [1 ]
Egorova, D. M. [1 ]
Stayno, L. P. [1 ]
Verbovska, S. A. [1 ]
Slynko, E., I [1 ]
Pichkur, L. D. [1 ]
机构：
[1] Natl Acad Med Sci Ukraine, State Inst Romodanov Neurosurg Inst, Kiev, Ukraine
来源：
WORLD OF MEDICINE AND BIOLOGY | 2021年 / 75卷 / 01期
关键词：
mesenchymal stem cells; human umbilical cord; suboccipital administration; spinal cord; pathologically altered neurons; demyelination; cryoprotectants;
D O I：
10.26724/2079-8334-2021-1-75-215-220
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Mesenchymal stem cells from human umbilical cord (1x10(6)) are able to correct neurodegenerative changes in the spinal cord of rats with chronic experimental allergic encephalomyelitis under the suboccipital administration. Cells cryopreserved in solution with 3 % dimethyl sulfoxide, 15 % ethylene glycol, 10 % sucrose, 12 % trehalose, 60 % fetal calf serum, slow the buildup of pathological changes at day 35; cryopreserved in solution with 4 % dimethyl sulfoxide, 6 % trehalose, 90 % fetal calf serum, - at days 35-60; cryopreserved in solution with 10 % dimethyl sulfoxide, 90 % fetal calf serum, - have no effect. Selection of optimal cryoprotectants to save the biological properties of these cells and working out the modes and ways of their administration remains relevant.
引用
收藏
页码：215 / 220
页数：6
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