Modulation of potassium channels via the α1B-adrenergic receptor in human osteoblasts

被引：7

作者：

Kodama, Daisuke ^{[1
]}

Togari, Akifumi ^{[1
]}

机构：

[1] Aichi Gakuin Univ, Sch Dent, Dept Pharmacol, Chikusa Ku, Nagoya, Aichi 4648650, Japan

来源：

NEUROSCIENCE LETTERS | 2010年 / 485卷 / 02期

关键词：

Noradrenaline; Human osteoblast; Sympathetic nerve system; alpha(1B)-Adrenergic receptor; Potassium channel; SYMPATHETIC-NERVOUS-SYSTEM; BONE-RESORPTION; ACTIVATION; EXPRESSION; DIFFERENTIATION; PROLIFERATION; STIMULATION; CURRENTS; SUBTYPE; PROTEIN;

D O I：

10.1016/j.neulet.2010.08.073

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Recent studies have demonstrated the involvement of the sympathetic nerve system in bone metabolism We have previously demonstrated the expression of adrenergic receptors in the human osteoblast SaM-1 cell line The aim of this study was to reveal the function of these receptors in osteoblasts using electrophysiological methods During whole-cell patch clamp recording, the application of noradrenaline reduced the currents Induced by a voltage ramp, and the effect was larger in the positive potential range Pretreatment with the selective alpha(1B)-adrenergic receptor antagonist chloroethylclonidine eliminated the inhibitory effect of noradrenaline Using Cs-based pipette solution to block potassium channels, the inhibitory effect of noradrenaline disappeared In contrast, the effect was unaffected in the presence of tetraethylammonium (TEA), a potassium channel blocker These results suggest that noradrenaline suppresses Cs-sensitive and TEA-insensitive potassium channels via the alpha(1B)-adrenergic receptor in human osteoblasts (C) 2010 Elsevier Ireland Ltd. All rights reserved

引用

页码：102 / 106

页数：5

共 36 条

[1] Chemical sympathectomy impairs bone resorption in rats: A role for the sympathetic system on bone metabolism [J].