A dual near-infrared pH fluorescent probe and its application in imaging of HepG2 cells

被引:107
作者
Tang, Bo [1 ]
Liu, Xia
Xu, Kehua
Huang, Hui
Yang, Guiwen
An, Liguo
机构
[1] Shandong Normal Univ, Engn Res Ctr Pesticide, Coll Chem, Jinan 250014, Peoples R China
[2] Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
关键词
D O I
10.1039/b707173f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A dual near-infrared pH fluorescent probe has been designed, synthesized and applied to HepG2 cells, with a pK(a) value of 5.14 under acidic conditions and 11.31 under basic conditions, which is valuable for studying acidic organelles in living cells and pH changes in chemical systems.
引用
收藏
页码:3726 / 3728
页数:3
相关论文
共 26 条
[1]   A pH sensitive fluorescent cyanine dye for biological applications [J].
Briggs, MS ;
Burns, DD ;
Cooper, ME ;
Gregory, SJ .
CHEMICAL COMMUNICATIONS, 2000, (23) :2323-2324
[2]   An efficient fluorescent probe for ratiometric pH measurements in aqueous solutions [J].
Charier, S ;
Ruel, O ;
Baudin, JB ;
Alcor, D ;
Allemand, JF ;
Meglio, A ;
Jullien, L .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (36) :4785-4788
[3]   Regulation and modulation of pH in the brain [J].
Chesler, M .
PHYSIOLOGICAL REVIEWS, 2003, 83 (04) :1183-1221
[4]  
Czarnik A. W., 1993, FLUORESCENT CHEMOSEN, P538
[5]   CHEMICAL COMMUNICATION IN WATER USING FLUORESCENT CHEMOSENSORS [J].
CZARNIK, AW .
ACCOUNTS OF CHEMICAL RESEARCH, 1994, 27 (10) :302-308
[6]   Signaling recognition events with fluorescent sensors and switches [J].
de Silva, AP ;
Gunaratne, HQN ;
Gunnlaugsson, T ;
Huxley, AJM ;
McCoy, CP ;
Rademacher, JT ;
Rice, TE .
CHEMICAL REVIEWS, 1997, 97 (05) :1515-1566
[7]   A novel acidotropic pH indicator and its potential application in labeling acidic organelles of live cells [J].
Diwu, ZJ ;
Chen, CS ;
Zhang, CL ;
Klaubert, DH ;
Haugland, RP .
CHEMISTRY & BIOLOGY, 1999, 6 (07) :411-418
[8]   MICROELECTRODE MEASUREMENT OF INTRACELLULAR PARAH OF MAMMALIAN HEART-CELLS [J].
ELLIS, D ;
THOMAS, RC .
NATURE, 1976, 262 (5565) :224-225
[9]   Synthetic macrocyclic peptidomimetics as tunable pH probes for the fluorescence imaging of acidic organelles in live cells [J].
Galindo, F ;
Burguete, MI ;
Vigara, L ;
Luis, SV ;
Kabir, N ;
Gavrilovic, J ;
Russell, DA .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (40) :6504-6508
[10]   CLATHRIN AND HA2 ADAPTERS - EFFECTS OF POTASSIUM-DEPLETION, HYPERTONIC MEDIUM, AND CYTOSOL ACIDIFICATION [J].
HANSEN, SH ;
SANDVIG, K ;
VANDEURS, B .
JOURNAL OF CELL BIOLOGY, 1993, 121 (01) :61-72