Slow vacuolar channels from barley mesophyll cells are regulated by 14-3-3 proteins

被引:50
作者
van den Wijngaard, PWJ
Bunney, TD
Roobeek, I
Schönknecht, G
de Boer, AH
机构
[1] Vrije Univ Amsterdam, Fac Biol, Dept Dev Genet, Bioctr Amsterdam, NL-1081 HV Amsterdam, Netherlands
[2] Univ Wurzburg, Lehrstuhl Bot 1, D-97082 Wurzburg, Germany
关键词
14-3-3; protein; barley mesophyll; patch-clamp; plant vacuole; slow activating vacuolar channel;
D O I
10.1016/S0014-5793(00)02394-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The conductance of the vacuolar membrane at elevated cytosolic Ca2+ levels is dominated by the slow activating cation selective (SV) channel. At physiological, submicromolar Ca2+ concentrations the SV currents are very small. Only recently has the role of 14-3-3 proteins in the regulation of voltage-gated and Ca2+-activated plasma membrane ion channels been investigated in Drosophila, Xenopus and plants. Here we report the first evidence that plant 14-3-3 proteins are Involved in the down-regulation of ion channels in the vacuolar membrane as well. Using the patch-clamp technique we have demonstrated that 14-3-3 protein drastically reduces the current carried by SV channels, The current decline amounted to 80% and half-maximal reduction was reached within 5 s after 14-3-3-addition to the bath, The voltage sensitivity of the channel was not affected by 14-3-3. A coordinating role for 14-3-3 proteins in the regulation of plasma membrane and tonoplast ion transporters is discussed. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:100 / 104
页数:5
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