Improved signal recovery for flow cytometry based on 'spatially modulated emission'

被引：3

作者：

Quint, S. ^{[1
]}

Wittek, J. ^{[2
,3
]}

Spang, P. ^{[2
]}

Levanon, N. ^{[4
]}

Walther, T. ^{[3
]}

Bassler, M. ^{[2
]}

机构：

[1] Saarland Univ, Dept Expt Phys, Campus E2-6, D-66123 Saarbrucken, Germany

[2] Fraunhofer ICT IMM, Carl Zeiss Str 18-20, D-55129 Mainz, Germany

[3] Tech Univ Darmstadt, Inst Appl Phys, Schlossgartenstr 7, D-64289 Darmstadt, Germany

[4] Tel Aviv Univ, Dept Elect Engn Syst, IL-69978 Ramat Aviv, Israel

来源：

METHODS AND APPLICATIONS IN FLUORESCENCE | 2017年 / 5卷 / 03期

基金：

欧洲研究理事会;

关键词：

flow cytometry; spatially modulated emission; digital filtering; pulse compression; SNR gain; RADAR; LABS;

D O I：

10.1088/2050-6120/aa7916

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recently, the technique of 'spatially modulated emission' has been introduced (BaSSler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. We present a comprehensive theory on SNR gain and provide experimental results of our concepts.

引用

页数：10

共 22 条

[1]

Ackroyd MH, 1982, RADIO ELECT ENG, V52, P309

[2]

Ackroyd MH, 1973, IEEE T AEROSP ELECT

[3]

[Anonymous], 2004, RADAR SIGNALS

[4]

Bassler M, 2008, Means for counting particles suspended in a fluid US Patent, Patent No. [0080181827A1, 0080181827]

[5] A NEW ALGORITHM TO OPTIMIZE BARKER CODE SIDELOBE SUPPRESSION FILTERS [J].

CHEN, XH ;

OKSMAN, J .

IEEE TRANSACTIONS ON AEROSPACE AND ELECTRONIC SYSTEMS, 1990, 26 (04) :673-677

[6] Efficient exhaustive search for optimal-peak-sidelobe binary codes [J].

Coxson, G ;

Russo, J .

IEEE TRANSACTIONS ON AEROSPACE AND ELECTRONIC SYSTEMS, 2005, 41 (01) :302-308

[7] Finding low autocorrelation binary sequences with memetic algorithms [J].

Gallardo, Jose E. ;

Cotta, Carlos ;

Fernandez, Antonio J. .

APPLIED SOFT COMPUTING, 2009, 9 (04) :1252-1262

[8] THE MERIT FACTOR OF LONG LOW AUTO-CORRELATION BINARY SEQUENCES [J].

GOLAY, MJE .

IEEE TRANSACTIONS ON INFORMATION THEORY, 1982, 28 (03) :543-549

[9] Monitoring CD4 in Whole Blood with an Opto-fluidic Detector Based on Spatially Modulated Fluorescence Emission [J].

Kiesel, Peter ;

Beck, Markus ;

Johnson, Noble .

CYTOMETRY PART A, 2011, 79A (04) :317-324

[10] Spatially modulated fluorescence emission from moving particles [J].

Kiesel, Peter ;

Bassler, Michael ;

Beck, Markus ;

Johnson, Noble .

APPLIED PHYSICS LETTERS, 2009, 94 (04)

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