Recent Approaches to Isolating and Culturing Mouse Bone Marrow-derived Mesenchymal Stromal Stem Cells

被引：7
作者：
Abdallah, Basem M. [1 ,2 ,3 ]
Khattab, Hany M. [4 ,5 ]
机构：
[1] King Faisal Univ, Coll Sci, Dept Biol Sci, Al Hasa 31982, Saudi Arabia
[2] Odense Univ Hosp, Dept Endocrinol, Endocrine Res KMEB, Odense, Denmark
[3] Univ Southern Denmark, Odense, Denmark
[4] Univ Hosp Wurzburg, Dept Internal Med 2, Div Mol Internal Med, Wurzburg, Germany
[5] Fayoum Univ, Fac Oral & Dent Med, Dept Prosthodont, Al Fayyum, Egypt
来源：
CURRENT STEM CELL RESEARCH & THERAPY | 2021年 / 16卷 / 05期
关键词：
Mesenchymal stem cells; BMSCs; cell culture; osteoblasts; cell expansion; haematopoietic progenitor cells; LONG-TERM CULTURE; PROGENITOR CELLS; IN-VITRO; CELLULAR SENESCENCE; GENE-EXPRESSION; SELF-RENEWAL; DIFFERENTIATION; POPULATIONS; INHIBITION; GROWTH;
D O I：
10.2174/1574888X15666200708134337
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The isolation and culture of murine Bone Marrow-derived Mesenchymal stromal Stem Cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodeling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of Bone Marrow-derived mesenchymal stromal Stem Cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with Haematopoietic Progenitor Cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.
引用
收藏
页码：599 / 607
页数：9
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