Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil

被引:9
作者
Reis, Rosani Santos [1 ]
Bonna, Isabel Cristina Fabregas [1 ]
Antonio, Isabela Maria da Silva [2 ]
Pereira, Sandro Antonio [2 ]
do Nascimento, Carlos Roberto Sobrinho [3 ]
Ferraris, Fausto Klabund [4 ]
Brito-Santos, Fabio [1 ]
Ferreira Gremiao, Isabella Dib [2 ]
Trilles, Luciana [1 ]
机构
[1] Oswaldo Cruz Fdn Fiocruz, Evandro Chagas Natl Inst Infect Dis INI, Mycol Lab, BR-21040900 Rio De Janeiro, Brazil
[2] Fiocruz MS, INI, Lab Clin Res Dermatozoonoses Domest Anim Lapclin, BR-21040900 Rio De Janeiro, Brazil
[3] Fiocruz MS, Natl Inst Qual Control Hlth INCQS, Mycol Lab, BR-21040900 Rio De Janeiro, Brazil
[4] Fiocruz MS, INCQS, Pharmacol Lab, Av Brasil 4365, BR-21040900 Rio De Janeiro, Brazil
关键词
cryptococcosis; domestic cats; MLST; molecular epidemiology; POPULATION GENOMICS; BRITISH-COLUMBIA; VAR; GRUBII; MATING-TYPE; GATTII; ANIMALS; FELINE; DOGS; MICROEVOLUTION; SUSCEPTIBILITY;
D O I
10.3390/jof7110980
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats.
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页数:11
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