MICROSATELLITE MARKERS IN THE AUSTRALIAN DESERT PLANT, SOLANUM CENTRALE (SOLANACEAE)

被引：3
|
作者：
Waycott, Michelle [1 ]
Jones, Brendan L. [1 ]
Van Dijk, Jent Kornelis [1 ]
Robson, Heather L. A. [1 ]
Calladine, Ainsley [1 ]
机构：
[1] James Cook Univ, Sch Marine & Trop Biol, Townsville, Qld 4811, Australia
来源：
AMERICAN JOURNAL OF BOTANY | 2011年 / 98卷 / 04期
关键词：
microsatellite; polyploidy; Solanum centrale;
D O I：
10.3732/ajb.1000356
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
引用
收藏
页码：E81 / E83
页数：3
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