Improvement of the Thermostability of Xylanase from Thermobacillus composti through Site-Directed Mutagenesis

被引：13

作者：

Tian, Yong-Sheng ^{[1
,2
,3
]}

Xu, Jing ^{[1
]}

Lei-Chen ^{[1
]}

Fu, Xiao-Yan ^{[1
]}

Peng, Ri-He ^{[1
]}

Yao, Quan-Hong ^{[1
]}

机构：

[1] Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

[2] Shanghai Ruifeng Agr Sci & Technol Co Ltd, Shanghai 201106, Peoples R China

[3] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2017年 / 27卷 / 10期

关键词：

Xylanase thermostability; Thermobacillus composti; disulfide bridge; structure; DISULFIDE BRIDGE; TRICHODERMA-REESEI; ACTIVE-SITE; THERMAL-STABILITY; GH11; XYLANASE; EXPRESSION; GENE; CLONING; IDENTIFICATION; STABILIZATION;

D O I：

10.4014/jmb.1705.05026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thermostability is an important property of xylanase because high temperature is required for its applications, such as wood pulp bleaching, baking, and animal feedstuff processing. In this study, XynB from Thermobacillus composti, a moderately thermophilic gram-negative bacterium, was modified via site-directed mutagenesis (based on its 3D structure) to obtain thermostable xylanase, and the properties of this enzyme were analyzed. Results revealed that the half-life of xylanase at 65 degrees C increased from 10 to 50 min after a disulfide bridge was introduced between the a-helix and its adjacent beta-sheet at S98 and N145. Further mutation at the side of A153E named XynB-CE in the C-terminal of this a-helix enhanced the half-life of xylanase for 60 min at 65 degrees C. Therefore, the mutant may be utilized for industrial applications.

引用

页码：1783 / 1789

页数：7

共 36 条

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