A secretory cellulose-binding protein cDNA cloned from the root-knot nematode (Meloidogyne incognita)

A cDNA encoding a secretory cellulose-binding protein was cloned from the root-knot nematode (Meloidogyne incognita) with RNA fingerprinting, The putative full-length cDNA, named Mi-cbp-1, encoded a 203 amino acid protein containing an N-terminal secretion signal peptide. The C-terminal sequence of the putative MI-CBP-1 was similar to a bacterial-type cellulose-binding domain, whereas the N-terminal sequence did not show significant similarity to any proteins in data bases. Recombinant MI-CBP-1 lacked cellulase activity, but bound to cellulose and plant cell walls, in Southern blot hybridization, Mi-cbp-1 hybridized with genomic DNA from M. incognita, M, arenaria, and M. javanica, but not M. hapla, Heterodera glycines, or Caenorhabditis elegans, Polyclonal antibodies raised against recombinant MwI-CBP-1 strongly labeled secretory granules in subventral gland cells of second-stage juveniles in indirect immunofluorescence microscopy, Enzyme-linked immunosorbent assay detection of MI-CBP-1 in stylet secretions of second-stage juveniles with the polyclonal antibodies indicated MI-CBP-1 could be secreted through the nematodes' stylet, suggesting that the cellulose-binding protein may have a role in pathogenesis.