Revised simulated body fluid

Preparation of a revised simulated body fluid (R-SBF) with ion concentrations, including those of Cl- and HCO3-, equal to those of the human blood plasma was attempted. Ion concentrations of the prepared SBF were confirmed to be equal to those of the blood plasma. The concentrations were unchanged at least for 4 weeks, when the R-SBF was sealed in a polystyrene container and stored at 36.5 degreesC. The R-SBF is believed to produce apatite with composition and structure equal to those of bone apatite, thereby being useful for in vitro assessment of bioactivity of a material and for biomimetic synthesis of bonelike apatite.