First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure

被引：50
作者：
da Silva, Cesar A. [2 ]
Oba, Eliane T. [1 ]
Ramsdorf, Wanessa A. [3 ]
Magalhaes, Valeria E. [5 ]
Cestari, Marta M. [3 ]
Oliveira Ribeiro, Ciro Alberto [4 ]
Silva de Assis, Helena C. [1 ]
机构：
[1] Univ Fed Parana, Dept Farmacol, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[2] Univ Fed Parana, Programa Posgrad Ecol & Conservaccao, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[3] Univ Fed Parana, Dept Genet, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[4] Univ Fed Parana, Dept Biol Celular, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[5] Univ Fed Rio de Janeiro, Ilha Fundao, Inst Biofis Carlos Chagas Filho, BR-21941902 Rio De Janeiro, Brazil
来源：
TOXICON | 2011年 / 57卷 / 01期
关键词：
Eutrophization; Traira; Cyanotoxin; Genotocity; Paralytic Shellfish Poison; PARALYTIC SHELLFISH TOXINS; POISONING TOXINS; CELL SHRINKAGE; ACCUMULATION; GLUTATHIONE; INHIBITION; TISSUE; SALMON; TETRODOTOXIN; QUANTITATION;
D O I：
10.1016/j.toxicon.2010.10.015
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Cyanobacterial waterblooms, such as the saxitoxin (STX) producer Cylindrospermopsis raciborskii, have been a worldwide concern in environmental health. However, the bioaccumulation of this neurotoxin in the trophic chain is not completely known. The aim of the present work was to evaluate STX bioaccumulation through chemical analyses and the toxic and trophic effects using biomarkers in the tropical freshwater fish Hoplias malabaricus. They were fed once every five days with Astyanax sp. before being subjected to intraperitoneal inoculation with SIX extract (0.08 mu g/100 g) obtained by lysis of toxic C raciborskii strain (T3). After 20 days the brain was collected for acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), lipoperoxidation (LPO), protein carbonylation (PCO), and comet assay analysis. The muscle was collected for STX chemical analysis. The activities of SOD and concentrations of PCO and LPO increased. The CAT, GST, and GPx activities decreased. Genotoxicity was observed in the experimental group. STX was not detected in muscle samples. Thus, an oxidative stress was observed in the brain, leading to the damage of lipids, proteins, and DNA. The mechanism of action of the neurotoxin in this subchronic exposure suggests an apoptotic cellular process. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：141 / 147
页数：7
相关论文
共 78 条
[41]  
Kao C.Y., 1993, P75
[42]  
KEEN JH, 1976, J BIOL CHEM, V251, P6183
[43]   The uptake, distribution and elimination of paralytic shellfish toxins in mussels and fish exposed to toxic dinoflagellates [J].
Kwong, Raymond W. M. ;
Wang, Wen-Xiong ;
Lam, Paul K. S. ;
Yu, Peter K. N. .
AQUATIC TOXICOLOGY, 2006, 80 (01) :82-91
[44]  
Lauterburg B.H., 1983, Drug Metabolism and Distribution, P166
[45]   Morphological abnormalities and sensorimotor deficits in larval fish exposed to dissolved saxitoxin [J].
Lefebvre, KA ;
Trainer, VL ;
Scholz, NL .
AQUATIC TOXICOLOGY, 2004, 66 (02) :159-170
[46]  
LEVINE RL, 1994, METHOD ENZYMOL, V233, P346
[47]   Retention and tissue damage of PSP and NSP toxins in shrimp: Is cultured shrimp a potential vector of toxins to human population? [J].
Linares, Jesus Perez ;
Ochoa, Jose Luis ;
Martinez, Ana Gago .
TOXICON, 2009, 53 (02) :185-195
[48]   Saxitoxin, a toxic marine natural product that targets a multitude of receptors [J].
Llewellyn, LE .
NATURAL PRODUCT REPORTS, 2006, 23 (02) :200-222
[49]  
MAGALHAES AC, 1931, MONOGRAFIA BRASILEIR
[50]  
Mortensen A.M., 1985, P165
12345678